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Preliminary study of Penicillium marneffei d1hA gene cloning and gene function

Author: ZhaoYunQian
Tutor: RenShuangXi；ZhaoGuoPing
School: Fudan University
Course: Microorganism
Keywords: Penicillium marneffei dlhA Histidine kinase Polar growth
CLC: Q93
Type: Master's thesis
Year: 2008
Downloads: 39
Quote: 0
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Abstract


The Penicillium Marneffei pathogenic fungi in a class of two-phase conditions at 25 ℃ culture presented saprophytic mycelium state , non- pathogenic , 37 ° C as early as now yeast state , pathogenic . Although it has a very important value of medical research and a unique temperature control of the two - phase conversion mechanism, but Penicillium marneffei genomic information and the lack of effective methods of operation and limitations . In our study , a 3936bp dlhA new genes have been successfully cloned . the dlhA and in the skin raw Bacillus gene found to play a global role drk1 highly conserved . We construct the RNA expression cassette , expression cassette having the target gene coding sequence of 430 bp inverted repeat sequence and is gus gene spaced . The entire interference vector with the Agrobacterium system successfully transformed Penicillium marneffei and in the controlled xylose the inducible promoter xylP regulation of the RNA interference . The study found dlhA gene silencing transformants morphology significant changes , and the loss of sporulation ability . The experimental results show that dlhA at different temperatures to adjust the different signaling pathways , has important implications for research polar growth , may be a two-phase conversion mechanism as well as drug targets for the screening .

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CLC: > Biological Sciences > Microbiology
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