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Objective: adenomyosis (adenomyosis) endometrial glands and stroma cells to myometrial benign infiltration and growth of disease. Adenomyosis is a benign disease, but its performance for the local cultivation and transfer, is considered a \The study showed that adenomyosis malignant etiology, histopathology and molecular genetics is closely related. In recent years, researchers have begun tumor from the point of view of the study of the etiology and pathogenesis of adenomyosis. Domestic and international studies have shown that the degradation of the extracellular matrix (extracellular matrix, ECM) is a key step of many malignant cell invasion and metastasis in tumor incidence. Abundantly clear that the family of matrix metalloproteinases (matrix metalloproteinase, MMPs) play a key role in the degradation of extracellular matrix, and almost all the ingredients in the degradation of ECM. Matrix metalloproteinase - 2 (matrix metalloproteinase-2, MMP-2), the main role is the degradation of the extracellular matrix of type IV collagen and gelatin endometrial extracellular matrix degradation, over-expression of MMP-2, to prevent endometrial cells to myometrial and stromal invasion and metastasis of structural damage, conditions occurred adenomyosis. RECK is newly discovered in recent years, the matrix metalloproteinase inhibitor, a variety of MMP expression can be suppressed at the posttranscriptional level, including MMP-2. Therefore, this topic choose MMP-2, RECK protein as detection indicators observed in control endometrium, adenomyosis eutopic endometrium and ectopic endometrial expression whether there are differences, explore RECK adenomyosis occurrence of the development of the relationship. Method: March 2007 - September archive at Qinhuangdao First Hospital, Department of Pathology, paraffin-embedded specimens: 30 cases of adenomyosis ectopic endometrium (hyperplasia, 15 cases, 15 cases of secretory phase) for the experimental group, eutopic endometrial hysterectomy due to adenomyosis in 30 cases (13 cases of proliferative phase, secretory phase 17 cases) and 25 cases of normal control group the intima (cases from other gynecological diseases and treatment of patients, mainly parovarian cysts, uterine mediastinum disease and tubal anastomosis endometrial adenomyosis patients of tubal surgery as the control group, the proliferative phase of the 12 cases, 13 cases of secretory phase). All patients age 35-57 years, mean age 46 years, no difference in the experimental group and the control group, the patient's age, preoperative March the patients were not taking hormone preparations for treatment, and other systemic diseases, postoperative specimen histopathology were confirmed by two pathologists diagnosed each group sections immunohistochemical staining under the microscope, MMP-2 and RECK staining intensity were recorded. Using the systems statistical software SPSS 11.5 statistical analysis. Results: 1 of MMP-2 in different periods in different endometrial expression: ectopic endometrium MMP-2 positive rate and secretory phase endometrium positive rates (P gt; 0.05), no statistically significant; the reign of MMP-2 positive rate and secretory phase endometrial proliferative endometrium positive rate (P gt; 0.05), no statistically significant; while the positive rate of control proliferative phase and the secretory phase endometrium positive rate (P lt; 0.05), there are significant secretory phase was significantly higher than the proliferative phase. RECK positive rate expression of 2.RECK in the different periods in the different endometrial: ectopic proliferative phase endometrium compared with secretory phase positive rate (P gt; 0.05), no statistically significant; eutopic endometrium RECK positive rate and positive rate of the secretory phase (P gt; 0.05), no statistically significant; the control proliferative phase endometrium RECK positive rate and positive rate of the secretory phase (P lt; 0.05), there are statistically significant, proliferative phase higher than the secretory phase (P lt; 0.05). 3. MMP-2 positive rate in a different endometrial expression: ectopic endometrium, eutopic endometrium and control endometrium in MMP-2 positive rate among the three groups had statistical significance (P lt; 0.05) . Comparison between groups drawn: not think positive expression rate of MMP-2 in ectopic endometrium and eutopic endometrium difference; ectopic endometrial MMP-2 positive expression that is higher than normal endometrium and The eutopic endometrial MMP-2 positive expression rate was also higher than that of the control intimal group. 4. RECK positive rates in different endometrial expression: ectopic endometrium, eutopic endometrium, endometrial tissue in control RECK significant positive rate. RECK expression in eutopic and control endometrium no difference between groups comparisons; ectopic endometrium and eutopic endometrium positive rate difference, the reign of the intima RECK expression is higher than ectopic endometrium; controlled endometrial with the ectopic endometrium positive rate more differences between the control endometrial RECK expression higher than the ectopic endometrium. Conclusion: of MMP-2 abnormal expression of adenomyosis incidence, the incidence of adenomyosis precipitating factor. 2.RECK loss of expression can lead to the occurrence of adenomyosis, RECK may prevent the occurrence of adenomyosis as a protective protein.
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