|
Objective: To investigate varicocele (VC) sperm cells in patients with human T -type calcium channel α1H and α1G genes relationship with varicocele , so as varicocele pathophysiology study provides a new direction for the the genetic disorder causes infertility diagnosis and treatment to find new ideas. Methods: According to the WHO standard method using computer-assisted semen analysis CASA semen taken for inspection, the results were divided into normal control group , VC semen normal group , VC abnormal sperm , and then use discontinuous Percoll gradient centrifugation to separate semen , the last semi-quantitative reverse transcriptase - polymerase chain reaction were detected by RT-PCR three groups semen T-type calcium channel α1H and α1G mRNA expression . Results: The three groups of sperm mRNA expression in two significantly different , α1H gene : (F = 8.25, P <0.01); α1G gene (F = 11.03, P <0.01). Compared with normal control group , VC normal group number two mRNA expression were decreased, but the difference was not significant (p> 0.05). Compared with normal control group , VC abnormal α1H gene expression was significantly decreased (1.08 ± 0.48), the difference was highly significant (Q = 10.03, P <0.01); VC abnormal group α1H mRNA expression were lower than VC normal group, the difference has a highly significant (Q = 6.54, P <0.01). Compared with normal control group , VC group α1G abnormal gene expression was significantly decreased (1.08 ± 0.48). Two specimens unexpressed , the results calculated by zero . Difference was highly significant (Q = 7.78, P <0.01); VC abnormal group α1G VC mRNA expression were lower than the normal group , the difference was highly significant (Q = 15.01, P <0.01). Human T -type calcium channel α1H and α1G gene in patients with varicocele amount of sperm expression Spearman rank correlation test , a positive correlation between the two (r = 0.59, P <0.05). Conclusion : Human T-type calcium channels and α1G α1H abnormal gene expression may lead to decreased semen quality in patients with VC \pathophysiological studies and subsequent gene targeted therapy provides a new direction.
|