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Purpose explore the transforming growth factor-β1 (Transforming growth factorβ1 of TGF-β1) induced after the autologous bone marrow mesenchymal stem cells (Bone Mesenchymal stem cells, BMSCs) combined with autologous biphasic bone matrix gelatin (Bone matrix gelatin BMG) original bit ported to the surgical removal of the rabbit intervertebral disc parts, observe the feasibility and effectiveness of the intervertebral disc repair. Method 1 48. Experimental Japanese rabbits were randomly divided into control group (n = 24) and the experimental group (n = 24), using in vitro cell culture technology, extracting the experimental group of animals iliac bone marrow into medium mixing centrifugal absorb seeded in culture after the the upper fat and supernatant bottle the primary culture medium was changed gradually remove non-adherent cells, to obtain the more pure adherent growth BMSCs2.BMSCs vitro purified passages were cultured, its morphological Observation of the growth curve and line CD34 of CD44 immunohistochemical identification of the BMSCs cultured to the third generation, TGF-β1 and vitamin C (VitaminC) induced differentiation, seed cells in tissue engineering, cartilage precursor cells, immunohistochemical their identification and observed induction effect to take the bone marrow of experimental group at the same time remove the ilium, rabbit iliac taken control group, in accordance with the the Urist method slightly improved degreasing, decalcified iliac deproteinized processing obtained after \scanning electron microscope produced rabbit intervertebral disc complete resection model, the experimental group - carrier complex, the control group were implanted autologous BMG implantation of autologous cells, respectively, in the first eight weeks, 12 weeks gross observation, HE staining, with sulfuric acid - carbazole azole method, RT-PCR detection proteoglycan, immunohistochemistry, RT-PCR was used to detect collagen type II. The data obtained by the SPSS 11.5 statistical software for statistical analysis. Results 1 with improved adherence screening method can obtain BMSCs, a purity better and by immunohistochemical identification of CD44-positive, CD34-negative; 2 in the induction of TGF-β1 and VitaminC BMSCs towards chondrocytes into get a lot of cartilage precursor cells, immunohistochemical staining positive; 3 BMG and electron microscopy of cells - carrier complexes visible BMG rough surface, multi-porosity, pore size is 100um ~ 800um, cells in the the BMG surface and pore inner wall grow well; 4 experimental group cartilage endplate intervertebral disc fibrocartilage tissue growth and repair, control group cartilage endplate and the fiber cartilage tissue repair is not obvious, eight weeks and 12 weeks experimental group cartilage endplate and fibrous cartilage aggrecan and Collagen II content compared with the control group were significantly increased (P lt; 0.05 ), the difference was statistically significant. Conclusion BMSCs intervertebral disc cell differentiation, proliferation, and repair intervertebral disc cartilage endplate and fibrous cartilage tissue.
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