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Objective: To measure the scar and normal skin thickness and Immunohistochemistry was used to detect each group scar and normal tissue substance P, P substance receptor hope through research organization of substance P, P substances receptor content and scar hyperplasia The relationship between the index and the preliminary study of substance P and its receptor stimulation scar formation mechanism to provide a theoretical basis for the prevention and treatment of scar. Method: a random sample of 24 cases of Plastic Surgery, scar resection of the Third Hospital of Hebei Medical University: hypertrophic scars in 9 cases, 11 cases of hypertrophic scars, keloids four cases, evenly divided between men and women, ages 10 years to 56 years. Each patient underwent a complete take the scar, and cut to take appropriate amount of surrounding normal skin, fixed, trimmed, dehydrated, paraffin-embedded specimens, vernier caliper measurement paraffin specimens of skin tissue thickness, calculated scar the proliferative index = scar thickness - normal skin thickness / normal skin thickness, unit: mm. Two pairs of specimens of tissue sections, a thickness of 5 μm, immunohistochemical staining of substance P in the organization, the nucleus hematoxylin dehydration Fengpian, and image acquisition, each specimen was measured gray value. Three pairs of specimens of tissue sections, a thickness of 5 μm, substance P receptor in tissue immunohistochemical staining, hematoxylin nucleus dehydration Fengpian, and image acquisition, determination of the gray value of each specimen. 4 the application sas9 statistical software, proliferative index, gray value groups, analysis of variance; proliferative index scar gray value, linear regression analysis. Results: 1 non-hypertrophic scar group, the hypertrophic scars groups keloid group average proliferative index were: -0.025233863, 1.802332415, 1.618440657. 2 optical microscope observed: control group skin structure is basically normal dermis fibers arranged in neat rows hypertrophic scars, arranged in parallel, we can see the structure of the normal gland, the dermal papilla evenly distributed, smooth curves, P substances and substance P receptors are mainly located in the basal layer of the epidermis and papillary dermis, nerve fibers and can also be found around the gland coloring; full layer structure compared with the normal atrophy, glands slightly reduce the fiber structure parallel arranged owe neat, P substances and the main coloring substance P receptors in the basal layer of the epidermis; hypertrophic scar dermal layer was significantly thicker glands to reduce the fiber structure disorganized, and was staggered the dermal papilla curve flattened; keloid dermal layer fiber structural disorder, we can see a large number of vortex structure, even visible structural disorder of the glandular structure papillary dermis, curve deepened and visible ring structure, the dermal papilla, a large number of mast cell infiltration, hypertrophic scars and keloids substance P and substance P coloring in the basal layer of the epidermis, the fiber structure of the gap and the remnants glands visible expression of substance P, fibroblast visible substance P receptor expression. 3 non-hypertrophic scar group, hypertrophic scar group scar keloid group P substance immunohistochemistry image analysis the average gray were: 101.2667,131.9636,132.325 P substance receptor immunohistochemical staining image analysis mean gray were: the normal skin P substance immunohistochemical image analysis 96.2358,130.6271,132.352; the hypertrophic scars Group, hypertrophic scar group, keloid group average gray values ??were: 109.0556,122.2182,126.95, P substance receptor The immunohistochemical staining average gradation of the image analysis respectively: 110.1362,121.5738,124.3571. The application the SAS9 statistics software analysis, substance P and substance P receptor content differences between scar group of non-proliferative scars and hypertrophic scars and keloids, no significant differences between hypertrophic scars and keloids; groups of normal skin There were no significant differences between substance P and the substance P receptor content. Paired t-test showed the substance P and substance P receptor image analysis results between the groups scar and normal skin, there are significant differences, which substance P hypertrophic scars scar image analysis gray value lower than normal skin, t = - 4.05, P = 0.00185, P substances the receptor image analysis grayscale value is also lower than normal skin, t = -4.5, P = 0.001, hypertrophic scars groups scar substance P image analysis gray values ??are higher than the normal skin, t = 7.02, P lt; 0.001; hypertrophic scar group scar substance P receptor image analysis gray values ??were higher than normal skin, t = 6.93, P lt; 0.001; substance P in the scar of keloid group image analysis of gray value higher than normal skin, t = 3.58, P = .01865; gray value of the substance P receptor image analysis were higher than normal skin scar keloid group, t = 4.54, P = 0.01. applications SAS9 statistical analysis software on non- group of hypertrophic scar, hypertrophic scar group keloid group three groups all data scar proliferation index and scar substance P image analysis gray value of the linear regression analysis (Table 6), the results show that there is no linear relationship, its scattered point is shown in Figure 11; hypertrophic scar group proliferative index and scar substance P image analysis gray value linear regression analysis (Table 7), the results indicate the existence of a linear relationship, the regression equation: y1 = -0.1973 0.0017x1 F = 65.14, P = 0.0002, scatter plot shown in Figure 12; the pathological scarring (hypertrophic scar group keloid combination and) proliferation index and scar substance P image analysis gray value linear regression analysis (Table 8), the results indicate the existence of a linear relationship, the regression equation: y2 = the -22.8784 0.1855x2, the F = 250, P lt; 0.0001 (Figure 13). Conclusion: 1 P substance is by stimulating dermal layer into the substance P receptor in the fiber cells and mast cells, promote scar hyperplasia role. 2 hypertrophic scars and keloids substance P and substance P receptor content was no significant difference compared with non-hypertrophic scars content. 3 hypertrophic scars degree of proliferation (proliferation index) a linear relationship between their substance P content; their substance P content of the degree of proliferation of pathological scar linear relationship; pathological scar proliferation index by substance P content. large non-hypertrophic scars.
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