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Objective: To establish the electrical stimulation of endothelial injury in rabbits thrombosis model to explore the seaweed polysaccharide on vascular endothelial injury induced inhibition of thrombosis, observing plasma activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT ) and ADP-induced platelet aggregation, thrombin an antithrombin complex (TAT), and vascular endothelial cell morphology, theoretical and laboratory based on the research and development of new antithrombotic drugs. Methods: 25 New Zealand rabbits were randomly divided into five groups, the normal control group, saline group, heparin group, the L01 low dose group, L01 high-dose group (n = 5). Each group using the same method to implement the common carotid artery, the intubation after, administered in the marginal ear vein. L01 low-dose group dose 2.5mg/kg; L01 high-dose group 7.5mg/kg; heparin group 1U/kg; normal control group and normal saline group were injected with saline and the first three groups of the same capacity. After administration 5min, in addition to the normal control group, the rest of the group were treated with electrical stimulation intravascular cause vascular endothelial injury induced arterial thrombosis record thrombosis, blood, on the other side of the artery after intubation determination of the rabbit plasma APTT, PT, TT and ADP-induced platelet aggregation rate. The enzyme-linked immunosorbent assay (ELISA), to detect the content of rabbit plasma TAT. Take electrical stimulation of segments of arteries, making biopsy and ultrathin electron microscopy sections, observe the vascular endothelial cell damage. Results: (1) Compared with normal saline, L01, the low-dose group can significantly prolong the rabbit arterial embolization time, and showed a dose-dependent effect. Compared with normal saline, L01, the low-dose group can significantly prolong the APTT, PT, TT, reduce platelet aggregation and to reduce the generation of plasma TAT and dose-dependent effect. (2) observed by light microscopy vascular morphology: the normal group intimal complete, smooth, clear boundary; the saline group intimal deficit fracture, damage deep grassroots to the subendocardial; heparin group, L01, low-dose group injury than saline group light. (3) electric microscope, the ultrastructure of the endothelial cells: endothelial cells of the normal group complete, clear boundary, abundant organelles; the saline group endothelial cells damaged deformation, fuzzy boundaries, organelles dissolution; heparin group, L01, low-dose group endothelial cell damage than those in the saline group light. Conclusion: L01 can inhibit a common pathway of endogenous and exogenous coagulation pathway - prothrombin activation or activity of thrombin, also can protect vascular endothelial cell injury, resulting in inhibition of thrombosis.
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