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The Relationship Rearch between Bax and Pathogenetic of Complicated Cataract Associated with Silicone Oil

Author: ZhangRong
Tutor: ShaoYan
School: Dalian Medical University
Course: Ophthalmology
Keywords: Silicone Lens epithelial cells Bax protein
CLC: R776.1
Type: Master's thesis
Year: 2011
Downloads: 18
Quote: 0
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Abstract

Objective: Apoptosis is also known as programmed cell death, is a distinguished from necrosis particular form of cell death, means triggered by the body and external factors stored in the intracellular cell death program which causes death mode initiative. With the relationship between apoptosis and disease research, and cataract lens epithelial cell apoptosis relationship has attracted people's attention. Recent studies show that the lens epithelial cell apoptosis is in addition to a variety of congenital cataract cataract outside the common cellular basis. With the development of technology vitreoretinal surgery, silicone oil as a vitreous filler has been used for complex vitreoretinopathy and achieved satisfactory results. Postoperative follow-up of silicone oil, silicone oil-filled eye cataracts as the most common complication, its incidence is almost as high as 100%. The development of cataracts can make the patient's visual acuity decreased again, but also affect the fundus examination, fundus and may delay diagnosis and treatment, therefore silicone oil filled eyes cataract occurrence, development ophthalmologist who has been more and more attention, but silicone oil causes The pathogenesis of cataract is not yet clear. This issue through Bax protein was observed in silicon oil eye cataract lens epithelial cells on the expression and function of lens epithelial cell apoptosis and explore the silicone eye concurrent fault correlation and pathogenesis of cataract caused by silicone oil. Methods: 45 cases of patients with different types of anterior lens capsule, including 20 patients with senile cataract 20, silicone eye cataract 18 patients 18 healthy adults all causes lens dislocation 7 patients 7, By HE staining and immunohistochemical methods Bax protein was observed in senile cataract, cataract and silicone intraocular lens dislocation of lens epithelial cells in patients with expression. Result: a light microscope lens epithelial cells in each group form: lens dislocation of lens epithelial cells uniform distribution, the same size, each chimeric cell nuclei were round, uniform size; senile cataract lens epithelial cells compared dislocation of the lens epithelial cells became smaller, the anterior capsule shrinkage, extremely irregular shape, with the capsule membrane separation, there is a cell enlargement, cell gap increased nuclear size and cell overlapping of inconsistencies; silicone eye cataract visible ago capsular curl, lens epithelial degeneration, fibrosis students, proliferation, cell shapes, multi Chengbian oval or elliptical, disorganized, and the cell gap to expand, some former subcapsular cell matrix see silicone oil droplets. 2 Immunohistochemical detection of bax protein in each group lens epithelial cells: bax protein in senile cataract cataract and silicone intraocular lens epithelial cells to express, in the lens dislocation of lens epithelial cells no expression, three groups bax protein expression between a significant difference (P <0.05), and the three groups also exist between any two statistical difference (P <0.05). bax protein in silicon oil eye cataract lens epithelial cells express stronger than senile cataract lens epithelial cells, there was significant difference between the two (P <0.05). Conclusion: Bax protein and silicone eye cataract occurrence and development of correlation; senile cataract and intraocular silicone oil may be related to the incidence of cataract lens epithelial cell apoptosis correlated, but both the pathogenesis may differ.

The Relationship Rearch between Bax and Pathogenetic of Complicated Cataract Associated with Silicone Oil

Abstract

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