Dissertation > Excellent graduate degree dissertation topics show

Preliminary Study on the Oxidative Damage and the Toxicity of Butyl Benzyl Phthalate(BBP)

Author: FangFang
Tutor: YuanJunLin
School: Central China Normal University
Course: Biochemistry and Molecular Biology
Keywords: butyl benzyl phthalate genetic toxicity oxidative damage DNA-protein crosslinks
CLC: Q504
Type: Master's thesis
Year: 2009
Downloads: 71
Quote: 1
Read: Download Dissertation

Abstract


Phthalate esters（phthalate esters PAEs）is the one of artificially synthesized organic compounds that has the biggest production and wide application,and it is a global environmental pollutant,widely existing in the air,water,soil and organism.Benzyl butyl phthalate(C19H20O4) is a sort of phthalateesters PAEs,which functions as plasticizer, softener,carrier and additive.It is classified as a important environment monitoring target by the world because of its anti-retrogradation,various pollution sources,high level of pollution,extensive toxicity,and causing various degree of danger to human health.This research is oriented on genotoxicity and oxidative damage of Benzyl butyl phthalate,and studies its molecular mechanism,which is of great significance for a full understanding of the genetic toxicity of BBP,and provides a certain experimental data for the study of the genetic toxicity of BBP and oxidative damage.My research is objected on mouse liver cell and Hela cell to study genetic toxicity of BBP by DNA-protein cross-linking experiments to explore the genetic toxicity,and study its oxidative-damage effect by means of detecting activity of SOD and MDA occupancy. Experimental results are as follows:1.BBP induced DNA-protein cross-linking effectUsing mouse liver cell and Hela cell as research material,the DPC induced by BBP was studied by application of SDS-KCl precipitate.Results showed that BBP could urge a production of DPC in mouse liver cell and Hela cell.Among them,the DPC effect at low concentrations is not obviously,only in the medium and high concentrations can cause significant DPC effect.2.BBP induced oxidative damage effectUsing intraperitoneal injection to mice in different concentration doses of BBP, determination SOD activity and MDA content of liver tissue cells,the effect of oxidative damage induced by BBP on mouse liver cells was detected.The results showed:SOD activity in liver cells has a declining trend with the increased concentration of BBP.when BBP exposure the concentration of 125mg/kg,the decreased Of the activity of SOD in the mouse liver cells ont obviously,there is no significant difference（P＞0.05）;and while BBP exposure in the concentration of 250mg/kg,500mg/kg and 1000 mg/kg,there is a very significant difference（P＜0.01）.With the increased concentration of BBP exposure, MDA content of liver cells in mice also increased,and compared with the control group, the MDA content of liver cells in mice increased one by one,and this increase has significant difference in concentration of 250mg/kg.This result showed the opposite trend coincides with the SOD activity effect of mouse liver cells caused by BBP.

Related Dissertations

  1. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  2. Influence of Baicalin on the Heart and Kidney Injury Induced by Iron Overload,R285
  3. Effect of Quercetin/hemeoxygenase-1/bilirubin System on Ethanol-induced Injury of Rat Hepatocytes,R285.5
  4. Heme oxygenase / carbon monoxide on alcoholic liver injury in rats and its mechanism,R575
  5. Protective Effect of Tetramethylpyrazine Diphenylmethyl Piperazidine on SH-SY5Y Cells Injured by Hydrogen Peroxide,R285
  6. Shou Tai Wan Reply TA Department of strain gene mutant experiment,R285.5
  7. The Toxic Effects of Octylphenol, Nonylphenol on the Reproductive System of F1 Generation Male Rats,R114
  8. Preliminary Study of Automobile Exhaust’s Toxic Impact on Rats’ Reproductivity and Genes,R114
  9. Castrated rat skeletal muscle mitochondrial oxidative damage and intervention studies,R285
  10. Oxidative Damage of Di(2-ethylhexyl)-phthalate (DEHP) to Gold Crucian Fish,X174
  11. The Preliminary Study of Molecular Mechanisms of Glutamate-induced Human Neuroblastma Cell Apoptosis and the Protective Effects of mGLP-1,Q255
  12. Study on Mechanisms of Testis Oxidative Injury and Apoptosis in Freshwater Crab (Sinopotamon henanense) Induced by Cadmium,X174
  13. The Effects of Garlic Oil on Lipid Peroxidation Induced by 2,5-Hexanedione and Metabolism of n-Hexane,R285
  14. The Effect of Probucol on Proliferation and Apoptosis of Vascular Smooth Muscle Cell Induced by Oxidation,R96
  15. Experiment Research of Endoplasmic Reticulum Stress on Oxidative Injury of Human Lens Epithelial Cells,R776
  16. α- lipoic acid on hydrogen peroxide -induced oxidative protective effect of cataract,R776.1
  17. An Empirical Study on Retinal Oxidative Damage of Blue Light after Cataract Surgery in Rabbit Modle,R774.1
  18. The Studies on the Damage Mechanism of Sub-Chronic Manganism in Cock’s Kidney,S858.31
  19. The Effect of Nitrie Oxide on Root Morphology and Antioxidant System in Pisum Sativum Seedings under Iron Stress,S643.3
  20. Analysis on Dune Reed’s Novel Content from Min Qin and Lin Ze and Protection of Protein Oxidation Injury,Q946
  21. Salicylate on paraquat induced lung injury intervention study,R595.4

CLC: > Biological Sciences > Biochemistry > General issues > Biochemical effects of
© 2012 www.DissertationTopic.Net  Mobile