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Black fungus [also known as fungus Auricularia the auricula (L.ex Hook) Underwood, light fungus, fine fungus, black dishes, belonging to the fungal door, Basidiomycetes, wood eyes and ears, fungus Branch, fungus genus, is a well-known drug Food used along with fungi. Black fungus is rich in glial good Qing slip faulting for the digestive system, in clear polyester gastrointestinal cellulose product lost food, textile, mining, and barber workers health food. Black fungus as the research object, a systematic study of the the black fungus collagen isolation technology, as well as different extraction methods on the the black fungus collagen features (such as emulsification, solubility, water absorption, oil absorption, water retention, etc.) impact. In this paper, three different methods were used to extract collagen separated from the black fungus, by comparison to determine the best method of separation. By single factor experiment and response surface analysis of experiments designed to determine the optimum conditions for hot water extraction of black fungus collagen: extraction time 6.71h, extraction temperature of 79.04 ° C, solid-liquid ratio 1:41.67, under optimal conditions, the black fungus collagen yield of 0.527 percent. Through the single factor experiment and response surface analysis of the experimental design to determine the optimum conditions: soda extract collagen black fungus that NaOH concentration 0.22mol / l, the extraction time 6.46h, solid-liquid ratio 1:58.82, in optimal conditions the black fungus collagen yield of 0.645 percent. By single factor experiment and response surface analysis experiment designed to determine the optimum conditions for alkaline protease extracted the black fungus collagen protein: 7.32% of the amount of enzyme extraction time 2.66h, solid-liquid ratio 1:52.63 in optimal conditions the black fungus collagen yield to 0.287%. As can be seen from the above data, the three methods, the Alkaline Extraction of in black fungus collagen protein was higher rate superior hot water method, and alkaline proteases. Drawn by the experimental data of this article, the hot water extraction of black fungus collagen firmness, consistency, viscosity, emulsion stability was significantly higher than that of alkaline and alkaline protease law Alkaline Extraction of the black fungus collagen emulsified oil absorption higher alkaline protease extracted the black fungus collagen adhesion index, water absorption, high water retention. Through the study of the black fungus collagen features, laid the foundation for its research in the fields of food, health products, cosmetics and other applications.
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