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Was to investigate the the 0.2% voriconazole sodium chloride solution used to clinical treatment otomycosis feasibility , its cochlear toxicity study . 20 white short-haired red guinea pigs were randomly divided into 4 groups: group of seven days , 14 days group , 20 day group and the normal group , n = 5 (10 ears) . Guinea pig detection ABR threshold in the ear microscope downstream bilateral tympanic membrane tension unit under large perforation , perforation detection threshold of ABR . Normal guinea pigs after testing decapitated bulla , paraffin section , HE staining and TUNEL apoptosis experiments , observe the vascular pattern, spiral ganglion cells and apoptotic index . The remaining three groups the middle ear topical 2mg/mL ( 0.2 percent ) voriconazole ear drops ear drops , in seven days , 14 days , after 21 days were decapitated take bulla Paraffin sections , HE staining and TUNEL withered death of experiment, observation stria vascularis , spiral ganglion cells in histological changes and apoptosis index . Perforation between the four groups of guinea pigs ABR Min value pairwise comparison , there was no significant difference ( p > 0.05 ) . Perforation ( before treatment ) , four groups of guinea pigs ABR threshold was pairwise comparisons , no significant difference ( p > 0.05 ) . Each group of guinea pigs before perforation after the perforation ABR threshold compared , a significant difference ( p < 0.05 ) . After treatment , the group of seven days , 14 days group , 20 day group ABR threshold its perforation ( before treatment ) showed no significant difference ( p > 0.05 ) ; pairwise comparison with the normal group, the ABR threshold , there was no significant difference ( p > 0.05 ) . The normal group, the group of seven days , 14 days group , 20 day group guinea pig cochlea HE staining clear vascular pattern , spiral ganglion cells with normal morphology . Four groups of guinea pig stria vascularis , spiral ganglion were seen in a small number of apoptotic cells , the group of seven days , 14 days group , 20 day group guinea pig stria vascularis , spiral ganglion cell apoptosis index were compared with the normal group , there was no significant sex difference ( p > 0.05 ) . Conclusion middle ear topical application of 2mg/ml ( 0.2 percent ) of voriconazole sodium chloride solution on the guinea pig ABR threshold value , stria vascularis and spiral ganglion cell apoptosis index has no effect , nor extend the treatment time change , indicating 0.2% The voriconazole sodium chloride solution, there is no cochlear toxicity .
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