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Epithelial ovarian cancer APE/Ref-1 expression of VEGF and MVD

Author: ZhangKuiMei
Tutor: JiXinQiang
School: Qingdao University
Course: Obstetrics and Gynaecology
Keywords: Ovarian Cancer Enzyme apurinic apyrimidinic endonuclease / redox factor-1 Microvessel density Vascular endothelial growth factor
CLC: R737.31
Type: Master's thesis
Year: 2009
Downloads: 36
Quote: 0
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Abstract


Objective: To investigate the relationship apurinic no pyrimidine endonuclease / redox factor -1 (APE/Ref-1) in the expression characteristics of epithelial ovarian cancer and vascular endothelial growth factor (VEGF) and angiogenesis. Methods: Immunohistochemical P-V6000 two-step method detected 62 cases of epithelial ovarian cancer, 30 patients with benign ovarian tumors and 20 normal ovarian tissue APE/Ref-1 and VEGF expression, the use of the vessel Nei Pite different markers CD34 marker and microvessel density (MVD), the final analysis of the relationship between APE/Ref-1 with VEGF and MVD. Results: (1) benign and malignant ovarian tumors and normal ovarian tissue in APE/Ref-1 expression, but the positioning is different. Only 20 cases of normal tissue in the nucleus and in the expression of 30 benign and 62 cases of cancer tissue nuclear expression exists, but four cases in the benign presence of cytoplasmic expression, cancer tissue in 48 patients with cytoplasmic expression. (2) compared to the ovarian cancer cell nucleus APE/Ref-1 Expression with normal tissue and benign no significant difference, while the cytoplasm APE/Ref-1 protein significant difference compared with the normal tissue and benign (P <0.05). Nucleus and cytoplasm APE/Ref-1 expression in ovarian cancer tissue with pathological grade, histological type Ⅲ ~ Ⅳ expression were significantly higher than in the clinical stage Ⅰ ~ Ⅱ stage. (3) three groups of VEGF expression significant differences (P <0.05) in the malignant group was significantly higher than the normal group and the benign group (P <0.05), VEGF expression between different pathological grades of ovarian cancer differences (P <0.05), and different clinical stage, histological type VEGF expression without significant difference. The study also found that massive ascites VEGF expression is significantly higher than a few. (4) benign MVD was significantly higher than the normal group (P <0.05), malignant group was significantly higher than that of the benign group. Compare different clinical stage I ovarian cancer MVD, the difference was statistically significant (P <0.05), and the different histological type, pathological grade MVD was no significant difference. (5) whether APE/Ref-1 cytoplasmic expression in ovarian cancer ovarian cancer is divided into two groups, a shift APE/Ref-1 cytoplasm of VEGF positive rate not APE/Ref-1 cytoplasmic expression by (P <0.05), MVD was significantly higher. Conclusions: (1) of APE/Ref-1 normal tissues only expressed in the nucleus, and the nucleus expression and nucleus cytoplasm of co-expression in benign and cancerous tissue, suggesting that the shift of our APE/Ref-1 cytoplasm may have some relation with the occurrence of ovarian cancer. (2) The ovarian cancer cell nucleus APE/Ref-1 Expression with normal tissue and benign tumors compared with no significant difference between the cytoplasm a APE/Ref-1 protein expression and compared to normal tissue and benign tumors were significantly different ( P <0.05). Prompted again our APE/Ref-1 cytoplasm shift may be associated with ovarian cancer. (3) The study also showed that the protein expression of of ovarian cancer APE/Ref-1 the presence of both in the nucleus or cytoplasm was increased with increasing clinical stage phenomenon the instructions APE/Ref-1 ovarian cancer and the prognosis may become effective indicators. (4) whether APE/Ref-1 cytoplasmic expression in ovarian cancer ovarian cancer is divided into two groups, a shift APE cytoplasmic expression of VEGF is high in not APE/Ref-1 cytoplasmic expression (P < 0.05), MvD also significantly increased, suggesting APE/Ref-1 cytoplasmic shift expression may be involved in the regulation of VEGF expression, thus contributing to ovarian cancer angiogenesis.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Ovarian tumors
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