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Experimental Study of the Expression of Cyclin G1 and NGF in the Process of Tissue Engineering Neural Graft Repair of Rat Sciatic Nerve Defect

Author: ZhangTao
Tutor: HanZhuang
School: China Medical University
Course: Surgery
Keywords: Tissue Engineering Cyclin G1 NGF Nerve injury Nerve regeneration
CLC: R651.3
Type: Master's thesis
Year: 2009
Downloads: 43
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Abstract

Purpose traumatic peripheral nerve defects is very common in clinical practice, in order to restore nerve function must restore nerve continuity. Rise of tissue engineering in the 1980s to provide a new direction for the repair of peripheral nerve defects. After chemically decellularized allograft nerve scaffold is a novel tissue engineering materials, studies have shown that promote nerve growth bone marrow mesenchymal stem cells (BMSc) grown in the the artificial neural bracket bridging nerve defects, nerve regeneration good role in promoting. Nervous system regeneration after injury mechanism is quite complex, nerve growth factor (NGF) promotes the survival and regeneration of neurons. Cyclin G1 is a new cyclin, its function is not yet fully understood, but studies have shown that Cyclin G family play a role in cell growth process the Morila found after nerve injury, monocyte neuron cells Cyclin G1 expression level was significantly higher in the process of nerve repair, the Cyclin G1 may play a role in the process of nerve cell regeneration. Rarely reported in the literature Cyclin G1 may be related to nerve regeneration. The experimental use of relevant animal models by SP immunohistochemical method to observe the Cyclin G1 and protein levels of NGF in artificial nerve bridge repair, explore CyclinG1 protein with artificial nerve bridge repair may be relevant for further research to provide experimental foundation. Materials and methods, specimens of tissue engineering artificial neural repair of peripheral nerve injury in animal experiments specimens. Method immune staining (SP) reaction three results acquisition and processing applications microscopic image analysis system results collection, statistical software SPSS10.0, results processing. Results of immunohistochemical staining results, CyclinG1 NGF protein expression the positive site located in the nucleus. CyclinG1 nerve stents, artificial neural and autologous nerve in the average gray value respectively 32.7072; 15.1750; 12.8776. NGF nerve stents, artificial neural and autologous nerve in the average gray value respectively 51.0900; 15.7400; 14.7110. Statistical processing results the prompt CyclinG1 and NGF in nerve scaffold repair process of peripheral nerve defects with the autologous nerve and artificial neural expression differences were statistically significant (p <0.05), visible CyclinG1 and NGF protein repair of peripheral nerve scaffold in tissue engineering increased expression in the process of nerve defects. Conclusion 1, Cyclin G1 protein expression increased, suggesting that Cyclin G1 may occur with nerve regeneration, development mechanism related process of peripheral nerve defects repaired nerve scaffold in tissue engineering in nerve regeneration stage. Nerve scaffold in tissue engineering in nerve regeneration stage repair of peripheral nerve defects course of NGF protein expression increased, suggesting that NGF may be related nerve regeneration occurred Development Mechanism.

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