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Effects of BMP-14 and bFGF on Proliferation in Rat Articular Chondrocytes: An in-vitro Research

Author: YuYao
Tutor: BaiXiZhuang
School: China Medical University
Course: Surgery
Keywords: Chondrocytes Bone morphogenetic protein -14 Basic fibroblast factor Type II collagen Proteoglycan
CLC: R684.3
Type: Master's thesis
Year: 2009
Downloads: 41
Quote: 0
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Abstract

Preface articular cartilage due to its lack of blood circulation system, relying on synovial fluid provides most of the nutrients, so once the damage is difficult to repair. Problems facing autologous chondrocytes as seed cells in cartilage tissue engineering research: slow the growth of cultured chondrocytes in vitro, can easily occur dedifferentiation meet tissue engineered cartilage construct that is difficult to obtain sufficient quantities required to maintain its normal phenotype of cartilage seed cells. Therefore, how in using appropriate concentration of growth factors and other cell factor of the joint stimulation by appropriate extension of chondrocytes body Gaiden passage number, there are conducive to obtain both a sufficient number and to maintain good table type of cartilage cells, to make autologous chondrocytes as cartilage tissue engineering seed cells become the focus of current research. Bone morphogenetic protein -14 (Bone morphogenetic protein, BMP-14) is a multifunctional growth factor, which is an important regulator of body growth factor, induce cartilage formation, promotion of bone, cartilage, tendon and ligament damage repair are important role. Fibroblast growth factor (fibroblast growth factor, FGF) stimulate chondrocyte proliferation and differentiation, and lead role mitogen morphogenesis. It is currently the strongest known to promote cell growth factor. The purpose of this experimental study BMP-14 and bFGF alone and combined to provide experimental evidence for the best combination of the different growth factor in chondrocytes in vitro proliferation and optimal concentration on cultured rat articular chondrocytes proliferation. The experimental method of in vitro isolation and culture of Wistar rat articular chondrocytes. Take good growth of the second generation of cartilage cells as the experimental cell packet distribution added to the different combinations and concentrations of growth factor. Group A was the control group, the experimental group based on the BMP-14 and bFGF concentrations in 15 groups, 16 groups. The each group BMP-14 concentration were taken 0ng/ml 50ng/ml, 100ng/ml, 200ng/ml and concentration of bFGF were taken 0ng/ml, 5ng/ml, 10ng/ml, 20ng/ml. 0, 2, 4 and 6 days cell count in the addition of growth factor, BMP-14 and bFGF proliferation. Expression of collagen type II using immunocytochemistry staining and semi-quantitative analysis, and image analysis software. Determination of proteoglycan expression alcian blue method. The experimental results of BMP-14 alone can promote the proliferation of chondrocytes in vitro, can not promote chondrocyte collagen type II and proteoglycan expression; of bFGF alone that can promote the outer cartilage cell proliferation, but also promote chondrocyte collagen type II and protein polysaccharide expression; role in promoting and combination effects are more obvious synergies. Conclusion This study confirmed that the combination of cultured chondrocytes in cartilage tissue engineering BMP-14 and bFGF promote chondrocyte proliferation is a viable method.

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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Joint disease and injury > Arthritis
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