|
Purpose:This experiment was based Traditional Chinese Medicine theory“Kidney essence the main bone marrow”as the basis. Development of Modern Science and Technology of the Chinese anti-osteoporosis drugs named JianGu Dropping Pills.Completed the Study of JianGu pills preparation、quality standards and othersMaterials and Methods: Orthogonal experimental design method were Epimedium and antler extract active ingredients, selected best of extraction; Using HPLC and UV spectrophotometry methods, research the quality standards to the conduct of Antler and Epimedium. For the JianGu Dropping Pills, optimization of the molding process by orthogonal design and studies on quality standards.Results:1 By orthogonal test method, the results of total protein extract as index, Determine the optimum antler extract for the total peptide substance is 8 times the amount of cache PH6.0 liquid extract 3 times, extraction time 45min.2 The Icariin content as a index,design the Orthogonal Experiment,compared different of extraction method by HPLC. Determine the optimum extraction technology Epimedium:Three Epimedium powder through sieve,plus 14 times the amount of 50% ethanol,Refluxing 3 times(3hours/time), filtration, Combined liquid filtration, concentration, drying.3 Preparation of the pills were investigated to determine the best preparation for the use of dimethyl silicone oil coolant: liquid paraffin = 3:1;Matrix PEG4000: PEG6000 = 2:3;The best conditions of dripping pills drug and matrix ratio of 1:2, the system drops the temperature 80℃, the coolant temperature of 15℃. 4 Quality standards: By HPLC on JianGu dropping pills several times in the index component determination, the standard curve in the range a good linear relationship, precision, stability, repeatability and recoveries are in line with standards.Conclusions: By epimedium, deer antler system science research, extract to determine the best extraction process, establish quality standards for Jiangu dropping pills and improved JianGu dropping pills study, For the further development and application of new drugs has laid a solid foundation.
|