Dissertation > Excellent graduate degree dissertation topics show

Characteristic Expression of cDNAs Induced during Late Zygotene-pachytene Stages of Microsporocytes in Lily Anthers

Author: WangShaoBo
Tutor: LiuHeng
School: Lanzhou University
Course: Cell Biology
Keywords: Lanzhou Lily Meiotic prophase I Suppression subtractive hybridization Reverse Northern dot blot hybridization Quantitative PCR
CLC: Q943
Type: Master's thesis
Year: 2009
Downloads: 85
Quote: 0
Read: Download Dissertation

Abstract


The plant meiotic prophase I is a very complex process , and late zygotene and pachytene is a critical period for completion of the homologous chromosomes Association and restructuring . Lanzhou lily as the research object , by suppression subtractive hybridization ( SSH ) method , separation and analysis of pollen mother cells in Lanzhou lily medicine late zygotene to pachytene significantly upregulated ESTs , using real-time quantitative PCR and the original in situ hybridization analysis of these ESTs corresponding to the gene expression profiles and the temporal and spatial expression characteristics , the purpose is to further understand the biological process of meiotic prophase I provide some useful information . The main findings are as follows : using suppression subtractive hybridization ( SSH ) analysis method to the specific the lilies drug development period - pollen mother cells in late zygotene to pachytene subtracted libraries for Tester in prior to Driver, and after two periods at the same time building a reverse library . Use reverse Northern dot blot hybridization to find positive clones screened out significantly up-regulated expression of 132 ESTs and sequenced , clustering and sequence assembly , get 34 ESTs stitching results , GenBank, BLASTN and BLASTX analysis , proved 34 there are the 18 sequences Cenbank database sequence ESTs with significant homology . Classification, in accordance with the MIPS classification criteria the reference GO classification criteria, the difference obtained expression ESTs were annotated and classification , will have significant homology ESTs related metabolic divided according to their function , protein modification , and signal transduction eight categories . Real-Time PCR analysis of the expression level of 12 ESTs , further quantitative research was supported by ESTs represent gene in meiotic prophase I late zygotene to pachytene anthers and other organizations expression . BH1 - 1 - high - frequency f9 ( LIM12 mRNA ) in the SSH library in situ hybridization analysis , combined with its Real-Time PCR data , initially revealed the gene expression patterns . We use the method of suppression subtractive hybridization screening in the prophase Ⅰ late zygotene to pachytene anthers upregulated ESTs, most of them unknown ESTs corresponding to the function of the gene is unclear, further research is needed for the study of meiotic prophase I provide some valuable reference information .

Related Dissertations

  1. Studies on the Quality of Phisio-Chemical and Fresh Keeping Technologies of Post-Harvest in Volvariella Volvacea,S646.13
  2. Analysis of Infrared Spectral Features and Identification of Al-responsive Genes in Small Black Soybean with Aluminum Stress,S529
  3. Gene Cloning and Expression Analysis of Odorant Receptor and Arginine Kinase Gene from Helicoverpa Assulta (Guenée),S433.4
  4. Construction and Primary Analysis of Differentially Expressed cDNA Library of Alfalfa by Using Suppression Subtractive Hybridization under the High Temperature,S541.9
  5. Analysis of the Differentially Expressed Genes in Fingered Citron under Cold Stress,S666.9
  6. Proliferation Characteristics of Type 2 Porcine Circovirus Strains and Antivirus Activity of Iprs and Aps on PCV2 in Vitro,S858.28
  7. The Study on Influence of Transcriptional Profiles of Porcine Peripheral Blood Lymphocytes Cultures Following Infection with Porcine Parvovirus,S858.28
  8. Freshwater planarian regeneration and Djnos eye point regeneration gene eye point correlation,X174
  9. Translocation Chromosome 1BL.1RS and Stripe Rust Resistant Varieties of Differential Gene Expression Analysis and the Study of Change in Physiological Parameters,S512.1
  10. Craniofacial Abnormalities in Zebrafish Induced by Clomiphene Sodium Citrate,R114
  11. GXLC porcine encephalomyocarditis virus pathogenicity of strains,S858.28
  12. Differential Proteomic Analysis and Research on DNA Methylation Transferase Expression of Silkworm Moultinism Variation,S882
  13. Molecular Cloning, Expression Analysis of Defence Related Genes from Citrus Infected by Ca. Liberibacter Asiaticus,S436.66
  14. Toll Like Receptor 2 and 9 Express in Peripheral Blood Monocyte of Chronic Severe Hepatitis B,R512.62
  15. Rice plant sulfur peptide hormone gene transfer system to build and Traits,S511
  16. The Construction of cDNA Libraries and Analysis of Differentially Expressed Genes at Maturation Stages of Ricinus Communis Embryo,S565.6
  17. Cloning and Salt-tolerance Research of V-H-ATPase Subunit Genes in Wheat,S512.1
  18. The Construcion and Analysis of Roots Suppression Subtraction Hybridization CDNA Library Before and After Tobacco Topping,S572
  19. The Expression of Bone Deficiency Factors in Osteoporosis Rats by Real-time Fluorogenetic Quantitative PCR,R580
  20. The Research of the Relationship between the Hepatitis B Virus Markers and HBV DNA in HBsAg Negative Serum,R512.62
  21. Screening, Full-length cDNA Cloning and Function Prediction of Cold-acclimation Differential Expression Candidate Genes in the Brain of Common Carp (Cyprinus Carpio),Q78

CLC: > Biological Sciences > Botany > Plant Cell Genetics
© 2012 www.DissertationTopic.Net  Mobile