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With the improvement of people's living standards, increasingly convenient traffic and changes in diet, making our rapid food-borne disease cases have increased in recent years, the types of zoonotic diseases are also increasing, the Guangzhou angiostrongyliasis which one. China has since the 1990s occurred in Beijing, Wenzhou, Yunnan and Fuzhou, Guangzhou Angiostrongylus small range outbreaks, among which the 2006 summer and autumn were up to more than 200 cases, particularly at night, causing the attention of the public fear and height. In this paper introduces a the Guangzhou angiostrongyliasis research status quo, the intermediate host of types and diagnostic techniques Research. Angiostrongylus cantonensis infection characteristics of the intermediate host snails in this study were identified from Guangzhou, Guangxi and Shanghai, Angiostrongylus for Angiostrongylus cantonensis PCR specific detection method for real-time PCR has short detection time, high sensitivity, and specificity characteristics, and several kill the apple snails drug preliminary laboratory study. Article shellfish perishables body Angiostrongylus cantonensis detection and wild with Phytophthora census provides a good diagnostic method, has important implications for food security, the prevention and control of Angiostrongylus cantonensis disease outbreaks and popular. The results are as follows: 1. Insect species identification: This test universal primers XZ1 NC2 as primers, respectively, collected from Guangxi, Guangzhou, Fuzhou and Shanghai seed tube round nematodes extracted the DNA as a template, amplified ITS- optimized conditions of temperature, primer concentration of about 250bp fragment, after sequencing results with Angiostrongylus cantonensis on Genbank sequence similarity of 99.5%, so the judge more than a few places Angiostrongylus Angiostrongylus nematode. 2 of the test for the first time established conventional PCR, the intermediate host of apple snails, giant African snail, white meat, snails in vivo method of Angiostrongylus cantonensis. Angiostrongylus cantonensis designed according to Genbank of Angiostrongylus cantonensis large subunit gene sequence-specific primers, optimized PCR conditions and results show that this method has a high specificity and sensitivity was significantly higher than the tissue homogenates method to achieve rapid, sensitive detection of the effect of the intermediate host of Angiostrongylus cantonensis. 3 In this study, for the first time successfully established TaqMan probes detect snails infected with Angiostrongylus cantonensis real-time PCR assay, conservative area designed TaqMan probes and primers, Mark mesogens and quencher, affecting optimized PCR reaction of a series of factors, the results establish the fluorescent PCR method sensitivity is much higher than that of conventional PCR detection methods with short time, high sensitivity, and specificity. This test studied the preliminary application effect of several drugs kill apple snails in the laboratory, through secret up to 6%, 6% Guanda laboratory research and new drugs 12.5% ??chlorinated salicylaldehyde amine different concentrations packet, records 2d, 3d, 5d 7d apple snails Deaths Statistics mortality, to determine the effects of three drugs molluscicide. The results showed that 6% of Cunard and new drugs 12.5% ??chlorinated the salicylaldehyde amine at the same concentration MOLLUSCICIDES effect slightly over 6% Smectite. Description of new drugs the chlorinated salicyloyl amine certain, certain restrictive laboratory needs further field testing to determine.
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