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Huperzia serrata China tends to endangered rare medicinal herbs, the thesis Huperzia serrata three different origins for the material, its cultivation adaptability, the active ingredient of Huperzine A and proteomics analysis, the results are as follows: resources survey, Huperzia serrata shade hygrophilous plants. Have higher shade degrees and high humidity environment conducive to the the Huperzia serrata growth; humidity high, the plants are growing robust, up to 20cm or more rapid growth, the number of small spores, low humidity, growing than weak about 10 ~~ 15cm, growth stunting, the scarce number of small spores. Soil survey results show that: the thick humus layer of sandy soil are more conducive to the growth of Huperzia serrata. Introduction and domestication results show that: the different introduction of Huperzia serrata plant significant difference in the survival rate of transplanted Huperzia serrata Daejeon County transplanting the highest survival rate was 65.5%. The lowest survival rate of transplanted Nanjing County Huperzia serrata, only up to 14.3%, and the growth potential adverse. Give shade the survival rate up to 77.8%, and growing well. Not give shade the survival rate of only 7.1%, growing adverse. The artificial cultivation instructions, enough canopy degree conducive to the Huperzia serrata transplanting cultivation. Loess texture cultivation survival rate, 75.0 percent, growing well; paddy fields, sandy soil survival rate of 18.8%, growing bad. In summary, the artificial cultivation of soil select better yellow soil, and need to be given the necessary shade; taken from Daejeon County Huperzia serrata adaptable. Huperzine A HPLC determination: WatersC18 column; flow rate of 1ml/min mobile phase: methanol: 0.08 mol / L ammonium acetate = 30:70, detection wavelength of 310nm, column temperature: 30 ° C, the injection volume : 10μl. The measurement results show that: the Huperzia serrata stem in three different regions of huperzine content greater than the content of the leaves, almost three times the content of the leaves. The content of leaves and stems are Daejeon County plant gt; Sanming suburb plants gt; Nanjing County plants, and each other to achieve a highly significant difference (P lt; 0.01). Huperzine A content Daejeon plant up leaves 87.537μg / g, stems for 197.796μg / g. Huperzia serrata three different origins proteomics research Huperzia serrata plant three different habitats protein electrophoresis pattern analysis using Imagemaser 2D Elite 5.0 image analysis software, a total of 15 different protein spots. 15 differential protein spots, 2,4,7,11 four points in Daejeon County plants expressing the highest abundance; 3,5,14,15 four points in Sanming outskirts plants expressing the highest abundance; collected from plants in Nanjing County, the other seven points in the protein expression of the highest abundance. Huperzia serrata reflect the different habitats in Fujian Province genetic diversity.
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