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Muscle contusion is a common type of trauma in the field of sports medicine, ranging from 10% to 55% of the incidence of skeletal muscle after injury healing a long time and many scar healing, seriously affecting motor function, is the field of sports medicine research hot and difficult. Studies have shown that skeletal muscle injury regeneration, but the limited capacity of skeletal muscle regeneration, structure and functional recovery is often incomplete, slow healing, and is accompanied by a certain degree of scarring, easy to injury again. The study found that early after skeletal muscle injury, damage local microvascular first destruction of a cell membrane damage, endothelial cell swelling, capillary permeability increase, peripheral vascular hemorrhage, thrombosis can not even control, leading to the degeneration of the membrane phospholipids, free based aggregation and inflammatory reaction induced oxidative stress, further exacerbating the damage to the surrounding normal skeletal muscle tissue. Domestic and international studies have shown that the microcirculation disorders and secondary tissue contusion caused the most serious damage 24h after injury. The local microcirculation exudate oxidation damage cells can lead to tissue reactive oxygen species (ROS) over-generation, ROS can further damage the lipid membrane thereby increasing skeletal muscle cell damage, and even necrosis, and antioxidant enzymes (superoxide dismutase, glutathione peroxidase, catalase) against oxidative stress injury protection in skeletal muscle cells. Therefore, how early to improve local microcirculation, reducing the formation of free radicals, enhance the activity of antioxidant enzymes, has become one of the hot spots of skeletal muscle injury repair. Our previous studies have to prove HuangqiDanshen injection promote skeletal muscle myosin heavy chain expression shorten the muscle healing process as well as improve the quality of healing role, but promote healing mechanism is not clear. The purpose of this study is to by the observed injection HuangqiDanshen oxidative stress and early impact of the microcirculation of skeletal muscle contusion explore HuangqiDanshen of injection to promote skeletal muscle healing process, whether the anti-oxidative stress, improve microcirculation, protection of skeletal muscle injury, and thus provide the experimental basis for the first part of the experimental study the effect of Danshen injection on oxidative stress in rats with acute skeletal muscle contusion after its further clinical application. Organization by measuring muscle contusion lipid peroxidation product malondialdehyde, antioxidant enzyme activity (superoxide dismutase, glutathione peroxidase, peroxide and catalase) to explore HuangqiDanshen injection whether it is by scavenging free radicals, increase the activity of antioxidant enzymes to promote healing after skeletal muscle injury in this experiment the second part of the study HuangqiDanshen injection on microcirculation after rat acute muscle contusion. By measuring the change of rats after systemic hemodynamics in skeletal muscle contusion, skeletal muscle wet, dry weight ratio, as well as changes in the diameter of the artery damage the local micro, capillary flow rate change, whether observation given Astragalus Danshen injection improve microcirculation, reduce swelling so as to promote the healing of damaged skeletal muscle. The first part HuangqiDanshen injection on oxidative stress in rat muscle contusion Objective: To observe HuangqiDanshen injection on oxidative stress in the repair process in rat skeletal muscle contusion: 72 male SD The rats were randomly divided into a treatment group, saline group, natural healing group (n = 24). The middle of the right gastrocnemius contusion model blow device caused damage local injection of astragalus Danshen injection, saline, not to deal with. 6 only blank control (without any treatment). Taken at 1, 4, 7 and 14 days after injury gastrocnemius content of malondialdehyde (MDA), the activity of catalase (CAT), superoxide dismutase (SOD) activity, glutathione peroxidase ( GSH-px) activity. Results: compared with the saline group and natural healing group, Chinese medicine group gastrocnemius SOD, GSH-px activity in 1,4,7 days was significantly higher (P <0.05), CAT activity did not change significantly (P> 0.05 ). Chinese Medicines gastrocnemius MDA content in 4,7 days after injury was significantly lower than the saline group and natural healing group (P <0.05). Conclusion: Astragalus Danshen injection can significantly reduce the MDA content of oxidation products, reduce oxidative damage in skeletal muscle contusion healing process, and significantly improve the activity of the skeletal muscle the reductase SOD and GSH-px. The second part HuangqiDanshen injection microcirculation after rat acute muscle contusion Objective: To observe The HuangqiDanshen injection of local microcirculation of the repair process in rat skeletal muscle contusion. Methods: 78 male Sprague-Dawley rats were randomly divided into a treatment group, saline group, natural healing group and blank control group. The first three groups to combat device caused the middle of the right gastrocnemius contusion model, were damaged local injection Astragalus Danshen injection, normal saline and injected drugs. Hours after injury, 24h, 72h, 120h gastrocnemius muscle in the body observed the microcirculation. Blank control group of normal rats, without any treatment, and the observation of the microcirculation as a control results: compared with the saline group and natural healing, Chinese medicine the treatment group microarteries diameter in 24h, 72h remarkable expansion (P < 0.05), and the capillary blood flow in 24h significantly increased (P <0.05). Compared with the blank control group, each group of wet / dry weight ratio (W / D) 2h, 24h, was significantly higher (P <0.05), but the treatment group were obviously lower than the saline group and natural healing group (P <0.05). Hemodynamics among the groups was no significant difference (P> 0.05) Conclusion: muscle contusion given early the injection HuangqiDanshen can significantly improve local microcirculation of injured skeletal muscle and reduce tissue edema, which reached to promote bone muscle injury healing
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