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The Expressions of Notch/Snail/E-Cadherin in Human Early Pregnancy Chorionic Villous Regulated by Mifepristone

Author: QianJinFeng
Tutor: HuangZiRong；FengYouJi；JinHongYan
School: Fudan University
Course: Obstetrics and Gynaecology
Keywords: Notch Snail E-Cadherin Mifepristone Early pregnancy Villus
CLC: R169.42
Type: Master's thesis
Year: 2009
Downloads: 63
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Abstract


Notch is an evolutionarily conserved transmembrane receptor protein family, can regulate cell proliferation, differentiation, apoptosis, development and angiogenesis the reconstruction aspects play an important role in embryo implantation; meters non-Division ketone as the widespread clinical use of the termination of early pregnancy drug, whether the mechanism of its anti-early pregnancy and the Notch signaling pathway, is not yet understood. This topic using RT-qPCR, immunohistochemistry method to detect phenotypic changes taking different doses of mifepristone, Villi Notch signal transduction pathway, to understand mifepristone as an anti-pregnancy drug, Notch signal transduction pathway. The topic is divided into two parts: (1) mifepristone Villi Notch-1 gene expression at the mRNA and protein levels; (2) mifepristone Notch downstream genes of Snail Villi, the E- Cadherin expression at the mRNA and protein levels of Notch ligands Dll-4 gene expression at the mRNA level. The first part of mifepristone Villi Notch-1 gene expression Objective: To investigate the 100mg, 150mg, 200mg three different doses of mifepristone in the mRNA and protein levels of Notch-1 gene expression Villi the impact. Methods: Requires normal early pregnant women to terminate pregnancy of 80 cases divided into four groups, Group 1 for the control group, the group of 2 to 4 preoperative mifepristone 100mg, 150mg, 200mg, 20 cases in each group. Detected using RT-qPCR Notch-1 mRNA expression in the four groups of induced abortion villi; Immunohistochemistry was used to detect the positioning of the Villi Notch-1 protein and semi-quantitative expression, to be compared with the control group. Results: Notch-1 mRNA and protein expression of different doses of mifepristone, 200 mg group role is most significant, and each dose group difference was significant (P <0.05), the 100 mg group expression changes. but the difference compared with the control group was not statistically significant (P> 0.05); 150mg group and the control group, the mRNA level difference was statistically significant (P <0.05), the difference was not statistically significant (P> 0.05) protein levels; 150mg and the difference between the 100mg two groups was not statistically significant (P> 0.05). Notch-1 protein to nourish cytoplasmic expression, interstitial little expression. Conclusion: Villi Notch-1 gene expression, and its expression was elevated after mifepristone 200 mg group, the role of the most significant (P <0.05). Suggesting that mifepristone may by regulation villi in the Notch signaling pathway involved in the mechanism of action of the anti-pregnancy. The second part of mifepristone on the the Villi Notch ligands and downstream gene expression of purpose: to explore the 100mg, 150mg, 200mg three kinds of different doses of mifepristone in the mRNA and protein levels of the Villi Notch signal turn pathway downstream gene expression of Snail, E-Cadherin Dll-4 mRNA expression level of Notch ligands. Methods: Case and grouped with the first part. RT-qPCR methods Snail, E-Cadherin, Dll-4 ERNA expression induced abortion Villi; using immunohistochemical methods to detect the villi of Snail, E-cadherin protein localization and semi-quantitative expression, Comparison with the control group. Results: (1) Snail gene expression, the expression of the mRNA levels of the Notch gene consistent, 200mg dose group significantly, there was a significant difference compared to other groups (P <0.05). 150mg, 100mg group changes, the difference was not statistically significant (P> 0.05); protein levels: 200mg and 150mg group and the control group significantly increased (P <0.05). 100mg group expression increased slightly, the difference was no significant difference (P> 0.05); trophoblastic nuclear expression of Snail protein expression mainly interstitial little expression. (2) the impact of mifepristone on the expression of E-Cadherin gene, mRNA levels dose group showed no significant difference (P> 0.05); significantly reduce the expression of E-Cadherin in the protein level of 200 mg mifepristone (P <0.05); 150mg and 100mg groups in varying degrees to reduce the expression of E-Cadherin protein, but the difference was not statistically significant (P> 0.05); small amount of interstitial cells express E-cadherin protein expression trophoblast membrane. (3) Dll-4 gene mRNA expression: after each dose mifepristone role, Villi Dll-4mRNA expression of slight changes, the difference was not statistically significant (P> 0.05). Conclusion: Villi Snail, E-Cadherin, Dll-4 gene expression, Snail gene expression increased after mifepristone, E-Cadhein decreased expression at the protein level, are the 200 mg group, the role of the most significant (P <0.05); the Dll-4mRNA group had no significant difference (P> 0.05); suggesting that mifepristone may by Notch / Snail / E-Cadherin signal transduction, regulation nourish the biological behavior of the cells, to participate in the anti-pregnancy mechanism of action.

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CLC: > Medicine, health > Preventive Medicine,Health > Family planning and health > Family planning methods > Interruption of pregnancy
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