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Identification and Expression in H Protein of Peste Des Petits Ruminants Virus (PPRV)

Author: HuiKai
Tutor: HuangZhiJian；ZhangBoQiang
School: Fujian Agriculture and Forestry University
Course: Veterinary
Keywords: Peste des petits ruminants virus Hgene Prokaryotic expression
CLC: S852.65
Type: Master's thesis
Year: 2009
Downloads: 63
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Abstract


In this paper,a primer was designed and synthesized based on the sequence of the nucleocapsid(H)protein genes of peste des petits ruminants virus(PPRV),which is added BamH I sites for amplifiting the Full-length fragment of the H gene from recombinant plasmids named p TOP-H using PCR.The fragment was cloned into pGM-T vector and transformed into E.coli TOP10,DNA sequence was determined.Then the cloned fragment was subcloned into prokaryotic expression vector pET-32a(+),and the recombinant expression plasmids were expressed in E.coli BL21(DE3) with inducement.The size of the recombinant fusion protein was 80ku(H) analysised by SDS-PAGE.The western-blotting results indicated that the expressed protein can be recognized by the PPRV positive serum.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Livestock Virology
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