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Extracellular Zn2+ Modulatory Effect on Inward Rectifing Potassium Channels Expressed on Carp Horizontal Cell

Author: ZhangYaZuo
Tutor: LiangPeiJi
School: Shanghai Jiaotong University
Course: Biomedical Engineering
Keywords: Retina Horizontal cells Inward rectifier potassium channel Zinc ions
CLC: R318.0
Type: Master's thesis
Year: 2011
Downloads: 3
Quote: 0
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Abstract


In the vertebrate retina, a large number of zinc ions present in the end of the photoreceptor cell axons and free of zinc ions together with glutamate is released into the synaptic cleft from the synaptic terminals of the photoreceptor cells. Retinal horizontal cells and photoreceptor cells synapse. In the dark, horizontal cell receives glutamate before the class of photoreceptor cells can enter, so speculate along with glutamate release zinc ions into the synaptic cleft may regulate ion channels expressed in horizontal cells. Retinal horizontal cells express a large number of ion channels, inward rectifier potassium channel (Kir) is one of them. In this paper, the whole-cell patch-clamp recording method, H1 type horizontal cells isolated carp retina to study the characteristics of the zinc ion regulation of Kir channels. First, we adopt the the Kir current on the step voltage stimulation or the sloping voltage stimulation to activate separation H1 horizontal cells, set the clamp voltage of -60 mV the the stimulus voltage range from -120 to 20 mV. Current inward rectifier characteristics, and may be subject to a high concentration (10 mM) Ba 2 blocking. Thereby indicating that the current is indeed mediated by Kir. Next, we examine the impact of zinc ions Kir. The experimental results show that, when the extracellular pH of the acidic environment (pH = 6.8), 2 mM of zinc ions generated Kir depressing effect. We also investigated 2 μM 20μM, 200μM, 1 mM, 5 mM, 10 mM, 20 mM of zinc ions on Kir. The results further demonstrate that when the zinc ion concentration of the concentration of the micro-molar level, zinc ion does not have any influence on the Kir; only when the zinc ion concentration mmoles level concentration, the zinc ions will Kir repressed, and with zinc ions the concentration, the greater the the degree of stress on Kir. Further, when the extracellular pH is acidic environment (pH = 5.0) or the value of the normal physiological range (pH = 7.2), whether it is a high concentration (2 mM) or a low concentration (2 μM) of zinc ion has no effect on Kir . These results suggest that zinc ions on the of Kir regulation of extracellular pH environment is closely related to, and has a concentration-dependent regulation of zinc ions Kir. We another part of the work has investigated the influence of the change of extracellular pH Kir. Experimental results show that, when extracellular pH decreased to 4.0, Kir been repressed. That Kir channels on the horizontal cells by extracellular pH regulation. In summary, our results show that (1) when the extracellular pH is acidic environment (pH = 6.8), milli-molar level concentration of zinc ions in the Kir channels having a depressing effect, while the micromolar concentration of zinc ions Kir channel has no effect; (2) when extracellular pH is acidic (pH = 5.0) or the normal physiological range of values ??(pH = 7.2), regardless of high concentration or a low concentration of zinc ions in the Kir channels are not affected. (3) The reaction characteristics of the extracellular pH changes can be adjusted Kir channels.

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