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Background: Hepatic veno-occlusive disease (hepatic veno-occlusive disease, VOD / HVOD) histologically characterized by thrombotic occlusion of the hepatic vein to hepatomegaly, right upper quadrant pain, jaundice and ascites as the main clinical manifestations . Efficacy thirty-seven Sanyu pain, qi blood, commonly used in the treatment of bruises, wounds bleeding, vomiting blood, the postpartum blood gas pain, and other diseases. The study found that soil notoginseng liver toxicity, soil notoginseng can lead pyrrolidine alkaloids (pyrrolizidine, PAs) the reason may HVOD soil notoginseng contains the pathogenic mechanisms are not yet fully clear. Reported in the literature to many cytokines involved development of HVOD liver fibrosis, for example, I L-6, IL-8, HSP-70. Prednisone, also known as dehydroepiandrosterone cortisone, prednisolone, prednisolone acetate, adrenocorticotropic hormone and adrenocorticotropic hormone drugs, anti-inflammatory, anti-allergic, anti-rheumatic, the immunosuppressive effects of prednisone therapy HVOD effective may inhibit the expression of cytokines, such as IL-6, IL-8, HSP-70, prednisone is by inhibiting the expression of these cytokines to prevent HVOD progress yet reported in the literature, this subject will be used immunohistochemical methods to detect soil thirty-seven Establishing HVOD the liver of a mouse model of IL-6, IL-8 and HSP-70 expression, explore prednisone whether through inhibition of IL-6, IL-8 and HSP-70 expression to prevent occurrence of HVOD, development. Methods: Mice our group previously completed gavage establish stable animal model the soil thirty-seven induced hepatic veno-occlusive disease, IL-liver line test the animals liver and prednisone intervention group of test animals by immunohistochemical methods 6, IL-8, HSP-70 detection. Animal model: 115 mice were randomly divided into four groups, soil notoginseng group of 30 (100g soil notoginseng boiled liquid, equivalent to a crude drug 1g/mL each mouse 30ml/kg/d to 0.6ml gavage, 30 days after treatment), the blank control group of 25 (PBS buffer 30ml/kg/d, each mouse to 0.6ml gavage for 30 days after treatment), a group of 30 (prednisone prednisone intervention piece in PBS diluted to 1mg/ml, each mouse was 5 mg / kg / d each 0.1ml gavage, with soil notoginseng 30 ml / kg / d orally at the same time, 30 days after treatment), strong Song intervention group 2 30 (prednisone tablets diluted to 1 mg / mL in PBS per mouse by 10 mg / kg / d each 0.2ml orally with soil notoginseng 30 ml / kh / d gavage at the same time, 30 days after treatment). Soil notoginseng decoction stable animal models to establish soil notoginseng induced hepatic veno-occlusive disease in mice orally. This experiment produced specimens from animal models of liver tissue biopsy, and through the two-step immunohistochemical method the mouse hepatic veno-occlusive disease in animal models and prednisone intervention group test animals the liver line of IL-6, IL-8 , HSP-70 detection, data using SPSS13.0 software package for statistical analysis. Results the: soil notoginseng mouse model meets HVOD performance light microscope soil notoginseng murine model of liver specimens, pathological features is also consistent with the pathological features of hepatic veno-occlusive disease. Immunohistochemical detection of results: soil notoginseng animal model mice liver tissue IL-6, HSP-70 levels were significantly higher than the control (30.99 ± 14.49vs93.42 ± 9.25,29.62 ± 1.91vs140.00 ± 12.68 P lt; 0.01), prednisone intervention group 1 and group 2, IL-6, HSP-70 levels are not elevated, and between the different dose intervention group had no significant difference (P gt; 0.05), while IL- 8 levels in PBS blank control group is the highest. Conclusion: In this study, soil notoginseng established decoction orally mice induced HVOD model by immunohistochemical detection of mouse liver IL-6, IL-8 and HSP-70 immunohistochemical detection comparator The model mice IL-6, the expression of the HSP-70 is significantly increased, suggesting that IL-6, HSP-70 may be involved in the pathogenesis of HVOD. The expression of IL-8 test results showed no significant change in the four groups, suggesting that IL-8 may not participate HVOD occurrence and development, but needs to be further confirmed. Prednisone intervention group (1,2), IL-6 and HSP-70 expression and blank control group no significant difference whether prompted prednisone treatment early HVOD through inhibition of IL-6 and HSP-70 expression, whether to participate to prevent the occurrence of HVOD development, its mechanism needs further research.
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