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Study on Antioxidants Combination of Different Levels as an Anti-oxidative Stress in Meat Duck

Author: WangCong
Tutor: DingBinYing
School: Wuhan Polytechnic University
Course: Animal Nutrition and Feed Science
Keywords: Oxidative Stress Tea polyphenols α-lipoic acid Silymarin Hydrogen peroxide , cumene hydroperoxide
CLC: S834
Type: Master's thesis
Year: 2009
Downloads: 130
Quote: 2
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Abstract

The test three antioxidants (tea polyphenols, α-lipoic acid, milk thistle hormone), different levels of combination of duck blood biochemical parameters, plasma and liver antioxidant indicators, meat quality. The test consists of four parts. The test an oxidative stress model to establish this test to be adopted to the duck intraperitoneal injection of hydrogen peroxide, cumene (CHPO), to explore the possibility of CHPO induced duck oxidative stress. The selection of the 42-day weight (3.36 ± 0.01), 20 kg of Cherry Valley Duck homologous pairing will be paired off, and then randomly assigned to two treatment groups, divided into stress group: the basal diet CHPO, control group: saline basal diet. Each group set up to 10 repetitions (duck). Saline the stress group duck injection of 30mg/kg · BW CHPO control group injected with equivalent. Continuous injection 5d, duck 50 days of age when blood collection. The results show that: (1) the injection CHPO after, plasma Cor levels, LDH activity increased (P lt; 0.01), CK activity increased (P lt; 0.05). (2) CHPO stimulate plasma H2O2 levels increased (P lt; 0.05) MDA level increased (P lt; 0.01). GSH-Px activity was reduced (P lt; 0.05). (3) CHPO stimulation resulted in plasma IL-6 levels increased (P lt; 0.05), IL-2 levels decreased (P lt; 0.01). Conclusion: injection CHPO lead duck generate oxidative stress, the body in the oxidative stress status. Test the combination of two different levels of antioxidants duck blood biochemical indicators of oxidative stress in this experiment to study the combination of different levels of polyphenols, alpha-lipoic acid and silymarin duck blood biochemical indicators, designed to explore its whether it can alleviate the oxidative stress caused by biochemical changes. The selection of 28-day-old healthy weight (1.76 ± 0.01) kg of Cherry Valley Duck 80 randomly divided into eight groups, each group set up to 10 repetitions (duck). Using a crossover design, three factors, two levels of each factor. Add a different level of polyphenols (100mg/kg and 150mg/kg) in the basal diet, respectively, α-lipoic acid (150mg/kg and 300mg/kg) and silymarin (100mg/kg and 200mg/kg), constitute a total 8 diets processing. From 42 days of age, to the test the duck injection of 30mg/kg · BW CHPO, continuous injection 5d, blood was collected in duck 50 days old when slaughtered. The results show that: (1) 150mg/kg tea polyphenols can reduce plasma GPT, GOT activity (P lt; 0.05). In lower plasma GOT vitality and GOT / GPT, tea polyphenols and α-lipoic acid interaction (P lt; 0.05) the three antioxidants may exist interaction (P = 0.099, P = 0.070). α-lipoic acid and silymarin in reducing plasma GOT / GPT interaction (P lt; 0.05). The 100mg/kg known as silymarin reduce GOT / GPT trend (P = 0.087). In the lowering plasma AKP vitality, tea polyphenols, α-lipoic acid and silymarin three antioxidants interaction (P lt; 0.05). (2) 150mg/kg polyphenols reduce plasma of LDH, CK vitality (P lt; 0.05, P lt; 0.01). Lower LDH activity in plasma alpha-lipoic acid and silymarin interaction (P = 0.093). Reducing plasma Cor levels, these three antioxidant tea polyphenols, α-lipoic acid and silymarin interaction (P lt; 0.05). 100mg/kg silymarin is known to reduce Cor-level trend (P = 0.081). The Conclusion: tea polyphenols for CHPO caused by changes in blood biochemical indicators better mitigation and better 150mg/kg effect. 100mg/kg silymarin their mitigation. Antioxidant indicators duck test the combination of three different levels of the antioxidants on oxidative stress in this experiment to study the combination of different levels of polyphenols, alpha-lipoic acid and silymarin on the duck plasma and liver antioxidant indicators aimed to investigate whether it can alleviate the oxidative stress caused by CHPO. Experimental design with test two. Each treatment group is determined when the end of the test plasma and liver antioxidant indicators. The results show that: (1) 150mg/kgα- lipoic acid reduces plasma LD and H2O2 level (P lt; 0.05), GSH-Px activity trends (P = 0.077). The 100mg/kg tea polyphenols reduce the H2O2 level in plasma (P lt; 0.01). 100mg/kg silymarin is known to reduce H2O2 level trend (P = 0.062). Polyphenols and α-lipoic acid in reducing plasma H2O2 level interaction (P lt; 0.05). (2) 300mg/kgα- lipoic acid improves liver T-AOC (P lt; 0.05). 150mg/kg polyphenols, 150mg/kgα- lipoic acid, 100mg/kg silymarin decreased liver MDA levels (P lt; 0.05). Three antioxidants in reducing the level of liver MDA interaction (P = 0.058). Conclusion: The three antioxidants help ease CHPO caused ducks oxidative stress, which this level of 150mg/kg polyphenols, 150mg/kgα- lipoic acid, 100mg/kg silymarin combination of the most help to reduce plasma LD and H2O2 levels and reduce the level of MDA in the liver. Experiment 4 combinations of the two different levels of antioxidants on oxidative stress the quality of meat duck choice of anti-oxidative stress effect on the basis of the test and test three, better two combinations studied whether it can alleviate the oxidative Stimulated adverse effects on meat quality. 28-day-old healthy body weight (1.85 ± 0.01) kg 40 Ducks were selected randomly divided into four groups, each group set up to 10 repetitions (duck). Test Group 1: basal diet (150mg/kg tea polyphenols 150mg/kgα- lipoic acid 100mg/kg water fly thistle) CHPO; experiment 2 groups: basal diet (150mg/kg tea polyphenols 150mg/kgα- lipoic acid 200mg/kg Silymarin) CHPO; CHPO Group: basal diet CHPO; control group: basal diet supplemented with saline. Start from 42 days of age, the injection of 30mg/kg · BW CHPO or saline, continuous injection 5d, a duck 50 days old when slaughtered test. Determination of the quality of the various groups of duck meat. The results show that: (1) CHPO stimulate test duck breast muscle pH0 value lower (P lt; 0.05) in the diets of tea polyphenols, the composition of the different levels of alpha-lipoic acid and silymarin improve CHPO stress Test ducks pectoral pH0 ??value (P lt; 0.05), reaches the level of the control group (P>; 0.05). (2) CHPO stimulate the duck breast muscle Hunter a value lower (P lt; 0.01) in the diets of tea polyphenols, alpha-lipoic acid and silymarin composition improve CHPO stress test duck breast muscle of the Hunter a value (P lt; 0.05), control group to achieve the level (P LT; 0.05). And test 2 Hunter a value higher than the control group and CHPO group (P lt; 0.01). The test test duck breast muscle Hunter L value than CHPO group decreased by 19.06% (P lt; 0.01). (3) CHPO stimulation resulted in trials duck breast muscle 24h elevated drip loss (P lt; 0.01) in the diets of tea polyphenols, alpha-lipoic acid and silymarin different levels of composition improve CHPO stress test duck breast muscle 24h drip loss (P lt; 0.01), and reached the level of the control group (P lt; 0.05). (4) the rate of water loss in the pectoral muscle, the test group 1 compared with CHPO group and the control group were reduced by 19.56% (P lt; 0.01) and 12.81% (P lt; 0.05). CHPO stimulation to cause the pH value in the pectoral muscle after thawing reduced (P LT; 0.05) in the diets polyphenols, the composition of the α-lipoic acid and silymarin different levels can increase the pH value (P LT pectoral after thawing; 0.01). Conclusion: CHPO stimulus can lead to a significant decline in duck meat quality, and in the diets of tea polyphenols, alpha-lipoic acid and silymarin composition of the different levels can mitigate its adverse effects on meat quality and test a group of better than test group 2.

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