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Hericium erinaceus (Hericium erinaceus) is part of Basidiomycota , Hericium erinaceus Division , the China's famous medicinal and edible fungus . It is rich in a variety of beneficial bacteria polysaccharides and essential amino acids and trace elements, with Lee five internal organs , aid digestion , nourishing and anti-cancer effects , mainly used to treat indigestion, gastric ulcer, gastric , duodenal carcinoma , gastric cardia digestive diseases and tumors. The Hericium erinaceus active substances research reported a lot , but most of Hericium erinaceus polysaccharide research on Hericium erinaceus oligosaccharides , especially the the Hericium erinaceus fruiting bodies of oligosaccharides is extremely rare . Oligosaccharide (Oligosaccharides), refers to a compound formed by the polymerization of 2-10 monosaccharide , it has improved lipid , hypoglycemic , anti-oxidation, to improve the intestinal environment and other biological activity , is widely used in food, feed , pharmaceutical and cosmetics industry . Oligosaccharide production using mainly transferase and hydrolase catalyzed glycosylation transfer reaction synthesis , reported obtaining oligosaccharides extracted directly from natural plant is not a lot , and the about edible fungi oligosaccharides research is very limited . Firstly, be separated by fractional alcohol precipitation method the fruiting bodies of Hericium extract , using the reducing sugar content of the respective components of the DNS reagent , thereby determining the Hericium oligosaccharides alcohol precipitation range , and found more than 85% alcohol precipitation in oligosaccharide -rich extract . And then by water extraction and alcohol Shen went polysaccharide obtained crude oligosaccharides , the yield was 2.75% , a relatively high concentration . Anion exchange chromatography (DEAE Sepharose FF) , and gel filtration chromatography (Bio-Gel P-2) and then the crude oligosaccharide separated to obtain 4 of the various components , respectively, relative to the salt phase component HEOF-SA and water component HEOF-WA, HEOF-WB , and HHEOF - WC . In order to determine the specific content and structure of each component by high performance liquid chromatography electrospray ionization tandem mass spectrometry and GC-MS analysis of the content and structure of each component , determine HEOF-WA a molecular weight of 5000 Da around the non- sugar components , slightly soluble in water ; HEOF-WB is a three - Ara oligosaccharides , the molecular weight of 414 Da , as white crystals , highly absorbent ; HEOF-WC is composed of arabinose and mannose 5.49 : 1 content in the composition of free monosaccharide mixture.
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