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Objective: To improve the CA / PEI membrane adsorption rate of of jaundice serum bilirubin (bilirubin, BIL), the experiment for the first time will be split the BIL and carrier albumin (albumin, ALB) complex method was applied to the CA / PEI film adsorption the BIL ago. Preparation of cholestasis and hemolytic jaundice canine model, access to the different nature of the jaundice serum CA / PEI film before and after the adsorption experiments compare joint split, total bilirubin (total bilirubin, TB), unconjugated bilirubin (conjugated bilirubin CB), unconjugated bilirubin (unconjugated bilirubin, UCB) and the concentration of ALB expedition split CA / PEI film Clear BIL impact. Method: 1. Establish the cholestatic jaundice dog model: two adult mongrel dogs, the application of bromocresol green method, vanadate and cyclic enzymatic determination of serum TB, CB, ALB and total bile acid (total bile acid, TBA concentration) infrastructure. Common bile duct end ligation and common bile duct - vena cava bypass method modeled after conventional feeding, anti-inflammatory, monitoring BIL concentration changes, respectively. Former postoperative every other day blood collection 30ml, a total of eight times, the latter postoperative daily blood collection 30ml of 6 times. 2. Establish hemolytic jaundice dog model: two adult mongrel dogs, the above method for determination of serum TB, CB, ALB and TBA basis of concentration. 3mg/kg and 5mg/kg injection standard the BIL solution. 30, 60 and 90 minutes after the blood 30ml. 3. Serum obtained jaundice were divided into three groups: group A film former group, as a control; membrane group, B group for over two hours of static adsorption through the membrane; C group split film group, to join appropriate amount of sodium deoxycholate split - phosphate buffer BIL, ALB, re-the above adsorption BIL; film after repeated measured biochemical indicators, comparing groups B, C clearance rate of TB, CB, UCB, and TB clearance rates were associated with TB concentration and ALB concentration. Results: 1. Jaundice animal models BIL change: common bile duct ligation and common bile duct - vena cava bypass method can be preparation of cholestatic jaundice model, former BIL concentration after upward - gentle - a downward trend, after 12 days of TB up to 134.9 μmol / L, 28 days after the BIL concentration dropped to a normal level, generally in good condition, with no complications. Which BIL concentration increased rapidly in a short period of time, six days after the TB up to 201μmol / L, but is generally in poor condition, died after one week. Hemolytic jaundice model standard BIL solution injection method can be prepared, the dog is no obvious abnormalities. Higher doses than the low dose of jaundice monitoring BIL concentrations within the same time increase, wherein UCB increased significantly, the two injection dose group the measured TB concentration were up to 105.8μmol / L, and 209.2μmol / L. 2 split on the film Clear cholestatic jaundice serum BIL: B, C group BIL have significant scavenging effect. C group compared to the clearance rate of TB, UCB Group B, a significant difference, P = lt; 0.01; CB clearance, no significant difference between the two groups, P gt; 0.05; clearance of the two groups of TB rate was positively correlated with TB concentration, respectively, r = 0.906 and r = 0.932; B group TB clearance rate was negatively correlated with ALB concentration, r = -0.624; the TB clearance rate of Group C with ALB concentration no significant correlation. Group C TBA concentrations were significantly elevated P lt; 0.01 3 split of the film clear hemolytic jaundice serum BIL: B, C group BIL have significant scavenging effect. Group C compared clearance rates of TB, UCB Group B there is a significant difference, P lt; 0.01; CB clearance, no significant difference between the two groups, P gt; 0.05; clearance rate in the two groups of TB TB concentration was positively related, r = 0.966 and r = 0.962; the B group TB clearance ALB concentration was negatively correlated (r = -0.816); Group C TB clearance with ALB concentration was no significant correlation. Group C TBA concentrations increased significantly, P lt; 0.01. Conclusion: 1. Bile duct ligation and common bile duct - vena cava bypass can establish dog cholestatic jaundice model, the former is easy to operate, no adverse reactions, but there is recanalization rate; latter surgical relatively complex, rapid formation jaundice but after a poor tolerance; the BIL intravenous injection can be established in a short period of time hemolytic jaundice dog model. 2. Join resolving agent sodium deoxycholate, CA / PEI film dogs cholestatic jaundice serum prepared using the above two methods, and hemolytic jaundice serum TB UCB showed better scavenging effect and The drop Yellow role from ALB concentration. But at the same time found that by the addition of sodium deoxycholate, TBA concentration was significantly elevated, raised new questions for further study.
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