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A Rat Model of Tibia Cancer Pain Produced by MADB-106 Mammary Gland Carcinoma Cells

Author: LiHuaYan
Tutor: YangBaoZhong
School: Shanxi Medical
Course: Anesthesiology
Keywords: cancer pain bone metastasis animal model
CLC: R738.1
Type: Master's thesis
Year: 2011
Downloads: 15
Quote: 0
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Abstract


Objective: To establish a tibial cancer pain model with MADB-106 mammary gland carcinoma cell line and to conduct therapeutic research through the behavior pain, x-ray, histological observation of the model.Methods: A rat model of bone cancer pain was established by intra-tibial inoculations of MADB-106 ratmammary gland carcinoma cells in Sprague-Dawley rats. Spontaneous pain was assessed by the reflection of Spontaneous paw withdraw ,move-evoked pain was observed by the extent of lower extremity claudication when the rats walked and heat hyperalgesia was evaluated by using a thermal dolorimeter . The structural damage of the bone was monitored by x-ray and histology.Results: The model group spontaneously withdrawed their paws 14.42±1.24 times on the 15 days after operation(P<0. 001), 18.33±1.37 on the 22 days(P<0. 001),21.25±1.54 on the 25 days(P<0. 001). The radiant pain thresholds of the model group was 11.86±1.63s on the 15 days after operation(P<0. 001),8.38±1.05s on the 22 days(P<0. 001),7.47为±1.25s on the 25 days(P<0. 001).The move-evoked pain scorn of the model group was 1.25±0.62 on the 15 days after operation(P<0. 001),2.00±0.95 on the 22 days(P<0. 001),2.33±1.07 on the 25 days(P<0. 001).The resuls showed that rats of the model group displayed the gradual development of Spontaneous pain ,heat hyperalgesia and move-evoked pain on the 15 -25 days after injection of the tumor cells, The x-ray of the tibia showed clear bone destruction. The histology of the bone showed the bone marrow cavitywas full of tumor cells, the cortical bone has been destroyed .Conclusion: This bone cancer model has been builded well and it will be useful to evaluate the therapy of cancer pain after two weeks .

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CLC: > Medicine, health > Oncology > Sports Department tumor > Bone tumor
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