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The first part of the Wistar rat C6 glioma model purpose: the establishment of a stable and reliable Wistar rat C6 glioma late stage model. Materials and Methods: C6 cells in containing 10? S, a mixture of 1% Green streptomycin, RPMI-1640 complete medium, placed in 37 ° C, 5% CO2 incubator culture, trypsin digestion, terminated acquisition the C6 cell count, the logarithmic growth phase detection cell activity, plus complete medium adjusted concentration. 2, using a random number table 22 Wistar rats were divided into an experimental group of 17, five of the sham group (control group). By stereotactic concentrations greater than 1.0x106.10μ1 C6 cells were inoculated to the experimental group, the right caudate nucleus and sham-operated rats injected in the same part of the complete medium. 3, approximately 3-4 weeks after tumor cell inoculation MRI scans and pathology observed corresponding rat. Results: 1, 17 rats were inoculated with tumor cells, one died of postoperative approximately 1h, 1 in the 15th after inoculation, unexplained death (subsequently pathologically confirmed tumor formation), the remaining 15 tumor cell inoculation rats in the subsequent MRI and pathological examination confirmed the formation of tumors, vaccination success rate of 100%. Sham group of five rats survived, and no tumor formation. 2, postoperative pathology confirmed by magnetic resonance and the end of the experiment after about 3-4 weeks after tumor cell inoculation presence of peritumoral edema and / or tumor cell infiltration. Conclusion: Wistar rat C6 brain glioma model reliable, rapid growth, inoculated with a high success rate, about 3-4 weeks after tumor cell inoculation meet peritumoral edema and / or tumor infiltration experiment requirements. The second part of the rat C6 glioma body 3.0T MR tensor imaging study purpose: To explore the 3.0T magnetic resonance tensor imaging in the Wistar rat C6 glioma tumor area and contralateral normal brain parenchyma to heterosexual fraction (FA) values ??and the average diffusion coefficient (MD) values, select a region of interest (ROI) and compare two different methods of measuring results. Materials and methods: random number table 22 Wistar rats were divided into an experimental group of 17, five of the sham group (control group). By stereotactic concentrations greater than 1.0x106/10μ1 C6 cells were inoculated to the experimental group, the right caudate nucleus and sham-operated rats injected in the same part of the complete medium. Approximately 3-4 weeks after tumor cell inoculation using 3.0T high-field-strength medical magnetic resonance scanner, rat dedicated coil rat C6 glioma model underwent conventional MRI, DT work T1WI and SAG T1-3D-bravo enhanced check, combined T2WI, FLAIR, T1WI and SAGT1-3D-bravo enhanced to determine tumor and edema, with the Function Tool software DTI data processing, to avoid the bleeding, necrosis, select more than three large (2mmm2, 9pix ) ROI and a single larger ROI, FA and MD values ??of tumor parenchyma and contralateral normal brain parenchyma. Results: 15 tumor formation in rats, the tumor area and contralateral normal brain parenchyma three other large average ROI FA were (0.1075 ± 0.02) and (0.312 ± 0.024), while the the three large ROIMD value were (0.944 ± 0.029) × 10-3mm2 / s and (0.808 ± 0.036) × 10-3mm2 / s, the two sets of results shown by the two-sample t-test differences were statistically significant (P <0.05). Tumor area and contralateral normal brain parenchyma single larger ROI FA average of (0.118 ± 0.039) and (0.310 ± 0.052), while the the single larger ROI MD average were (0.941 ± 0.047) × 10-3mm2 / s and (0.806 ± 0.043) × 10-3mm2 / s, the two sets of results shown by the two-sample t-test difference was also statistically significant (P <0.05). Two different methods to select tumor area the ROI obtained the FA values ??by the paired t-test showed a statistically significant (P lt; 0.05), and MD values ??was not statistically significant (P> 0.05). Conclusion: The clinical 3.0T MRI line of rat C6 glioma DTI scans practicable, by measuring the tumor area, contralateral normal brain parenchyma FA and MD values, provides a useful reference for future research work. Methodology, the study found that two different methods to select the ROI impact on the FA value measurements, and no significant impact on the MD value measurements. The third part of AQP1 expression in the rat brain C6 glioma peritumoral edema infiltrating organizations FA value research purposes: research the Wistar rat C6 glioma peritumoral edema infiltration organizations magnetic resonance diffusion tensor imaging ( MR-DTI) characteristics and immunohistochemistry water channel protein 1 (AQP1) controls investigate AQP1 expression in glioma tumor tissue edema infiltration parameters part of the MR-DTI fractional anisotropy (FA) related sex. Materials and methods: random number table 22 Wistar rats were divided into an experimental group of 17, five of the sham group (control group). By stereotactic concentration greater than the 1.0 × 106/10ul C6 cells were inoculated to the experimental group, the right caudate nucleus and sham-operated rats injected in the same part of the complete medium. Approximately 3 to 4 weeks after tumor cell inoculation, 3.0T high field strength of medical magnetic resonance scanner rats coil rat C6 glioma model underwent conventional MRI, DTI, FLAIR, T1WI and SAG T1-3D-bravo enhanced MRI combined T2WI FLAIR.T1WI and SAG T1-3D-bravo enhanced to determine tumor and edema, with the area of ??the the Function Tool software DTI data processing, to avoid hemorrhage, necrosis, and affect the outcome, select multiple 3 other large (2mm2, 9pix) ROI, the corresponding parts of the FA values. Let tumor contralateral normal brain tissue as an internal control. Immediately after the completion of the MRI scan line 4% paraformaldehyde fixed whole brain, after excess of 1% sodium pentobarbital, the rats were sacrificed whole brain fixed the peritumoral edema brain tissue, the maximum level of the selected and DTI tumor corresponding do consecutive coronal sections, HE staining and AQP1 antibody immunohistochemistry check. IOD values ??of the Pearson correlation analysis detected FA values ??and the corresponding parts AQPl positive expression correlation, calculate correlation coefficients, t-test, P lt; 0.05 as statistically significant. Results: 15 tumor formation in rats, peritumoral edema about 3-4 weeks 3 ROI FA mean value (0.204 ± 0.036), compared with the contralateral normal brain tissue the multiple ROI FA average (0.310 ± 0.027 ) decreased, but not significantly lower tumor two sets of results show that the difference was statistically significant (P <0.05) by two-sample t-test. Pearson correlation analysis, the FA value AQP1 positive expression of the infiltration of tumor edema organizations IOD values ??between negative correlation (r = -0.81, P lt; 0.05). Conclusion: the Wistar rat C6 glioma peritumoral edema infiltration Organization MR-DTI parameters of FA values ??with AQP1 negative correlation between AQP1 positive expression increased FA values ??decrease.
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