Dissertation > Excellent graduate degree dissertation topics show

Inhibition and regulation of miR-22 on ovarian cancer invasion and metastasis

Author: LiJun
Tutor: LuXin
School: Fudan University
Course: Obstetrics and Gynaecology
Keywords: Ovarian cancer Invasion Metastasis miR-22 Tiaml
CLC: R737.31
Type: Master's thesis
Year: 2011
Downloads: 136
Quote: 0
Read: Download Dissertation

Abstract


Objectives. This work is aimed to identify the regulatory effect of miR-22 on cell migration, invasion, growth and apoptosis in ovarian cancer, and to make a general analysis of miR-22’s downstream functional targets with an attempt to gain a better understanding of miR-22’s role in ovarian cancer.Methods. (1)We examined the distinct miRNA expression profiles between paired low-metastatic human serous ovarian cancer cell SKOV-3 and high-metastatic human serous ovarian cell SKOV-3ip using miRNA microarray. Particularly, Real-time RT-PCR was performed to validate miR-22 expression level in paired SKOV-3/SKOV-3ip cells and HO-8910/HO-8910PM cells respectively. (2) Gain-of-function study and loss-of-function study was respectively performed in SKOV-3ip cells, with a relatively higher expression level of miR-22, and SKOV-3 cells, with a relatively lower expression level of miR-22, to monitor the changes in cell invasion and migration, cell growth, and cell apoptosis. (3) Three bioinformatics databases(TARGETSCAN, http://www.targetscan.org, MIRANDA, http://www.microrna.org and PICTAR, http://www.pictar.mdc-berlin.de) was employed to screen the potential targeting genes of miR-22. In particular, we validate the regulatory effect of miR-22 on Tiaml expression by dual-luciferase reporter assay, Western Blot and Real-time RT-PCR.Results. (1) Validation by Real-time RT-PCR revealed that miR-22 was decrease in SKOV-3ip cells and HO-8910PM cells, compared with their counterparts, SKOV-3 cells and HO-8910 cells, respectively. (2) Both gain-of-function and loss-of-function study displayed an negative effect of miR-22 on cell invasion and migration in vitro without significantly affecting cell viability and apoptosis. (3) Subsequent bioinformatics analysis revealed that miR-22 might regulate multiple pro-metastatic genes. Particularly, regulatory study of miR-22 in Tiam1 expression was performed. Our results showed that Tiam1 was a direct target of miR-22, revealed by dual-luciferase reporter assay, and alteration of miR-22 expression had a negative regulatory effect on Tiam1 protein level and mRNA level, demonstrated respectively by Western Blot and Real-time RT-PCR.Conclusions. Taken together, our findings suggested that miR-22 might be involved in inhibiting ovarian cancer metastasis, probably by targeting Tiam1.

Related Dissertations

  1. The Regulate and Controlof Slug on P53-MDM2 System,R730.2
  2. Effect of Invasive Plants (Conyza Sumatrenss and Alternanthera Philoxeroides) on Soil Carbon and Nitrogen Processes,X173
  3. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  4. Effects on Telomere Length of Epithelial Ovarian Cancer by Chemotherapy,R737.31
  5. The Contribution of the CCl22, CCR4 in the Milky Spots Peritoneal -metastasis of Gastric Cancer,R735.2
  6. Recurrent Ovarian Cancer Surgery Again with Surial Analysis of Relevant Factors,R737.31
  7. Old Ⅲ Colorectal Cancer Survival after Radical Analysis and Evaluation of Chemotherapy,R735.3
  8. Clinical Analysis of Postoperative Recurrent-metastatic Gastrointestinal Stromal Tumors,R735
  9. Surveys of Short-term Efficacy of TP Therapeutic Regimen Applied to 81 Cases of Ovarian Cancer Patients,R737.31
  10. Expressions and Relationships of PI3K/AKT and Apoptosis Related Regulation Factors in Epithelial Ovarian Tumors,R737.31
  11. The Study on Expression of CLIC1 on Lymphatic Metastasis of Mouse Hepatocarcinoma,R735.7
  12. The Location and Expression in Mouse Heptocarcinoma Cell Lines with High and Low Lymphatic Metastatic Potentials.,R735.7
  13. The Study of the Correlation of Prognosis and Pattern Evaluation of Patients with Recurrence of Rectum Cancer,R735.37
  14. α1, 3 fucosyl transferase Ⅳ (Fut4) upregulated increased metastatic potential of squamous cell scc12,R730.2
  15. CXCR1/CXR2 receptor antagonists on mouse ascites hepatoma lymphatic metastatic potential impact,R735.7
  16. Anti-tumor Effect of Trastuzumab on Nude Mice Xenografts of Human Ovarian Cancer SKOV3 Cells and Its Synergistic Effect of Action with Docetaxel, Cisplatin,R737.31
  17. Relationship between Epithelial-mesenchymal Transition Induced by Hypoxia and Invasive Ability in Hepatocellular Carcinoma Cells,R735.7
  18. The Role and Mechanism of SDF-1/CXCR4 Axis in Small Cell Lung Cancer Invasion and Metastasis,R734.2
  19. The Influence and Mechanisms of Mythylglyoxal on Growth and Apoptosis of Human Ovarian Cancer Cells HO8910,R737.31
  20. The Animal Study of Biological Active Peptide RGDS Inhibits Colorectal Cancer from Liver Metastases,R735.34
  21. The Study of Risk Factors and the Correlation of NF-κB and GM-CSF with Bone Metastasis in Breast Cancer,R737.9

CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Ovarian tumors
© 2012 www.DissertationTopic.Net  Mobile