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Investigation of the Prevalence of Japanese Encephalitis B in Zaozhuang Prefecture, Shandong

Author: ZhouChuanLing
Tutor: ZhouHongNing
School: Dali University
Course: Pathogen Biology
Keywords: Japanese encephalitis Vector infection rate JE antibody levels Shandong Zaozhuang
CLC: R181.3
Type: Master's thesis
Year: 2011
Downloads: 28
Quote: 0
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Abstract


Objective: To clarify Zaozhuang encephalitis vector species composition, mosquito encephalitis infection rate situation and the local situation antibody levels in healthy population encephalitis , Japanese encephalitis prevention in the region to provide an effective basis . Methods: The survey methodology mosquito species , taken at the observation point pen overnight mosquito mosquito lamp mosquito collection methods , the captured mosquitoes for taxonomic identification to ascertain the local mosquito species composition ; encephalitis virus mosquito infection rate methods of investigation , after identification of the captured mosquitoes in frozen aliquots by category tube, frozen in liquid nitrogen and placed in a laboratory to take isolated and identified by RT-PCR of Japanese encephalitis virus ; healthy population encephalitis antibody level survey methods, observation point near the village respectively 1.5-20 years old and 20 years of age both groups blood (2ml / person ) , centrifuged serum made ??in the laboratory using ELISA kit IgG antibody positive cases of Japanese encephalitis results : A total of capturing two subfamilies 4 , 7 species 34,174 mosquito , Culex among three main advantages of belonging to the local mosquito species, accounting for 92.84 percent of total mosquitoes captured ( 31730 / 34174 ) ; take the RT-PCR method for separation of flavivirus the positive rate of 38.1% in the batch , from three Culex isolated six Japanese encephalitis virus , natural infection rate of 1.75/10 Wan , the sequencing of the Japanese encephalitis virus belongs isoform ; were collected from 278 sera from healthy people , encephalitis IgG antibody positive rate was 41.4% ( 116/ 278 ) , where gt; 20 -year-old age group of Japanese encephalitis antibody positive rate was significantly higher than 1.5 years old -20 years age group (χ2 = 11.786, P lt; 0.05) female population JE workers gG antibody levels than men (χ2 = 6.641, P lt; 0.05), not vaccinated JE vaccine vaccinated population was higher than the number of JE vaccine crowd -positive rate ( χ2 = 13.215, P lt; 0.05) Conclusion: the first time in Zaozhuang Japanese encephalitis virus isolated genotype 1 , three Culex encephalitis major part of the local media , local encephalitis higher prevalence , Tip the local epidemic prevention departments should strengthen encephalitis vaccination , and make mosquito control measures.

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CLC: > Medicine, health > Preventive Medicine,Health > Epidemiology and Prevention of Disease > Epidemiology of the basic theory and methods > Special epidemiological each
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