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Objective To investigate the bone marrow mesenchymal stem cell transplantation in the treatment of acute spinal cord injury mechanism . Method 1. Prepared using modified Allen 's fight against the rat T10 spinal cord injury model , the sham group 48 injury group, 96 were randomly divided into a control group and transplantation group , each group was further divided into one days after transplantation , three days 7 days , 14 days , four subgroups , each subgroup of 12 rats . Seven days after the injury , the control group and transplantation group were injected via the tail vein PBS suspension 1ml phosphate-buffered saline (PBS) 1ml and rat bone marrow stromal stem cells turn green fluorescent protein (GFP-BMSCs) single cell ( 1x106 BMSCs). After transplantation 1 day, 3 days, 7 days , 14 days , injury motor function in rats applications BBB score evaluation before and after treatment to improve the situation . 3 and 14 days after transplantation , application of light microscopy and electron microscopy the morphological changes of the spinal cord tissue ; transplant after 3 days , 7 days , the application of fluorescence excitation light observed BMSCs migration in vivo survival . Transplant recipients 1 day , 3 days , 7 days , 14 days , the application of immunohistochemistry to detect damage in rats of Nogo- A and SYN expression . Results 1. Motor function of the injured rat transplant group and the control group had varying degrees of recovery. The damage zone of the transplant group spinal cord structure as compared with the control group , relatively complete , showing obvious motor function improvement ( P LT ; 0.05 ) . The transplant group visible GFP-labeled cells in the damage zone and its surrounding area was gathered and survival . 3 . Transplant after 3 days , 7 days and 14 Nogo-A expression in the spinal cord injury area surrounding tissue transplantation group than in the control group was significantly lower ( P lt; 0.05 ) . 4 transplant after three days , the first 7 days and 14 days , spinal cord injury area surrounding tissue transplantation group SYN expression was significantly higher ( P lt; 0.05 ) compared with the control group . The Conclusion 1.BMSCs by peripheral vascular transplant can penetrate the blood-brain barrier , and damage to the spinal cord region migration , and promote the recovery of neurological function in rats with acute spinal cord injury in its memory alive differentiation . 2.BMSCs inhibit transplantation in the treatment of acute spinal cord injury damaged local Nogo-A protein expression , reducing glial scar formation and promote axon regeneration . 3.BMSCs transplantation in the treatment of acute spinal cord injury is conducive to increased expression of the damaged local synaptophysin (SYN) , to promote the recovery of synaptic function .
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