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A Study on the Treatment of Spinal Cord Injury by Transplantation of Human Umbilical Cord Mesenchymal Stem Cells at Different Time

Author: WangYing
Tutor: FengShiQing
School: Tianjin Medical University
Course: Surgery
Keywords: Spinal cord injury Umbilical cord mesenchymal stem cells Cell transplantation Different periods Rats
CLC: R651.2
Type: Master's thesis
Year: 2011
Downloads: 54
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Abstract

To observe spinal cord injury after transplantation of human umbilical cord between the different periods of local mesenchymal stem cells (human umbilical cord mesenchymal stem cells, hUCMSCs) the effect of the repair of spinal cord injury explore hUCMSCs partial transplantation in the treatment of spinal cord injury (spianl cord injury SCI) most Jia transplant timing. 【Methods】 80 female Wistar rats were randomly divided into groups A, B, C, D, n = 20, prepared to Impactor Model-Ⅱ blow thoracic spinal cord 10 (T10) injury model. The A group CMW group Group B after injury the 3d transplant hUCMSCs, C group damage after the 1w transplantation hUCMSCs the D group injury after of 3w transplantation hUCMSCs, after the modeling 24h, one day before transplantation and after transplantation 8w (groups A and D group corresponding time points after injury) week hindlimb function of rats in each group behavioral ratings (BBB score); 8w immunohistochemical staining observed after transplantation survival and differentiation of transplanted cells and vascular regeneration 10w with biotin dextran amine (biotin dextran amine, BDA) tracer corticospinal tract (corticospinal tract, CST) the observed axon regeneration. [Results] The SCI after 24h of each group of rats BBB score without significantly with difference (P gt; 0.05), injury 3w transplant hUCMSCs group 1w with simple injury group before transplantation and after transplantation corresponding time points of BBB rated no noticeable with sex difference (P gt; 0.05), 2w each time point score after transplantation were significantly higher than the corresponding time points CMW group, the difference was statistically significant (P lt; 0.05): CMW group after SCI 9w reach the plateau Compared with the end of the experiment, the difference was not statistically significance (P gt; 0.05). BBB score after transplantation transplantation gradual increase in the previous point in time after a point in time with the same group difference was statistically significant (P lt; 0.05) after transplantation the 6w start after injury 1w transplant hUCMSCs group BBB rated significantly higher than 3d the transplant hUCMSCs group and injury after injury the 3w to transplant hUCMSCs group (P lt; 0.05). Immunohistochemical staining observed no positive Brdu cells, vascular morphology of the damage zone incomplete spinal cord CMW group, no neovascularization; damage surrounding astrocyte proliferation, cell hypertrophy processes increased, irregular arrangement, forming glial scar; no BDA mark in the corticospinal tract through the damage zone. Injury 3d the transplant hUCMSCs group, 1w to transplant hUCMSCs group and injury after injury 3w spinal cord transplantation hUCMSCs group in both Brdu labeled cell survival 1w to transplant hUCMSCs group and injury after injury, the number of cells in the 3w the transplant hUCMSCs group, significantly more than 3d the transplant hUCMSCs group after injury, but were not seen in the three groups of transplanted cells differentiate into neurons or glial-like cells; 3d the transplant hUCMSCs group and injury after injury 3w transplant hUCMSCs the group damage around the astrocytes than CMW group decreased, but the arrangement still owe the rules; injury 1w transplant hUCMSCs group damage around astrocyte morphology become normal alignment rules, and there are a large number of astrocytes through the damage zone, number of angiogenesis by injury The area of ??the corticospinal tract the nerve fiber significantly 3d the transplant hUCMSCs group and injury after injury 3w Portable hUCMSCs group. [Conclusion] after SCI the 1w local transplant hUCMSCs not only long-term survival, can significantly inhibit glial scar formation, improve microcirculation, so as to promote axonal regeneration, improve the experimental hindlimb motor function. Tips and 3d and 3w, SCI 1w better time to choose hUCMSCs local transplantation in the treatment of SCI.

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CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord
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