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Identifying of Microbes Contaminated in Long-term Cultivation of Plasmodium Falciparum in Erythrocytic Stages in Vitro and Research the Methods of Controlling the Quality of Laboratory Atmosphere

Author: LiYuGang
Tutor: DongYing
School: Dali University
Course: Pathogen Biology
Keywords: Plasmodium falciparum cultivation in vitro identifying of microbes quality controlling of laboratory atmosphere
CLC: R378
Type: Master's thesis
Year: 2011
Downloads: 12
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Abstract


Objective First, to search the microbes species which contaminated in culture of Plasmodium falciparum in vitro and the sources, drug susceptibility, as well as uncover the methods of prevents and controls. Second, research of the effects of disinfection for laboratory atmosphere with ultraviolet rays irradiation and methanal fumigation. Methods 5% carbon dioxide mixture atmosphere was poured into the culture dish and incubating P. falciparum in 37 centigrade temperature. Identify if the culture medium was contaminated with microbes. Identifying of the microbes on the operator’s hands was by surface sampling method. Then, enhanced the disinfection by mopping the floor with 84-disinfectant, scrubing the doors and operations area with bromogeramine and prolonging the radiation time of ultraviolet rays everyday, and washing hands with bromogeramine before operation, as well as sprayed ethanol to the surfaces of materials in operational process. Analysis of laboratory air samples were by plate natural sedimentation method before and after different time length of ultraviolet rays irradiation (one to four hours); as well as taking one sample before and seven samples (24hours interval everyone) after methanal fumigation, then repeated them after two weeks. Results The microbes which contaminated culture medium were Sernotrphomonas maltophilia-、A. baumannii / calcoaceticus complex and Acinetobacter Iwoffii (acilwo)。They are Gram’s negative bacillus and multiple drug resistance strains. The microbes on the operator’s hands were common strains as S. epidermidi and M. luteus. The component of microbes in air samples varied in different phases. On the early stage, the majority of microbes were bacillus such as Pseudomonas,A. baumannii and E. cloacae. On the late stage, the majority were Gram’s positive coccus as M. luteus and S. saccharolyticus, which were sensitive for gentamycin sulfate. Methanal fumigation and prolonging the radiation time of ultraviolet rays could decrease the number of microbes in laboratory atmosphere. Conclusions Strengthen disinfection and utilization of gentamycin could effectively avoid of microbes contaminating when culturing of P. falciparum in vitro. Various factors could impact the effects of ultraviolet rays and the results of air samples by plate natural sedimentation method. The curve about number of microbes in laboratory atmosphere changing with different radiation time of ultraviolet rays could not depict accurately and easily. Methanal fumigation could well decrease the number of microbes in laboratory atmosphere, however, the effects weakened gradually with time extending, and the odour nuisance might touch the health of operators within 24-48hours after fumigation.

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CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Pathogenic bacteria
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