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The Influence in AMCC’s Structure and Function by Serum of Asthmatic Rats and the Intervene Effect of Kidney-tonifying Recipe

Author: ZouJunZuo
Tutor: HuChengPing
School: Central South University
Course: Department of Respiratory Medicine
Keywords: Asthmatic rats serum chromaffin cells Kidney-tonifying recipe epinephrine treat asthma from kidney
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 7
Quote: 0
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Abstract


BACKGROUDAim of the study:the aim of the study was to observe the effect of asthmatic rats serum on the function and structure of the AMCC,and investigate the channel of the treatment of asthma from kidney theory by kidney-tonifying recipe. Methods:Thirty two Sprague-dawley rats were divided into four groups in random(n=8):control group(CG), asthmatic group(ASG), dexamethasone group(DXMG) and kidney-tonifying group(KTG). CG, ASG and KTG rats were sensitized and challenged with ovalbumin (OVA) rather than control rats. Some another Sprague-dawley rats collected adrenal medulla for primary culture of chromaffin cells. Then, divided into four groups in random:control serum group(CSG), asthmatic serum group(ASSG), dexamethasone serum group(DXMSG) and kidney-tonifying serum group(KTSG). Each group Then the cells intervene by experimental rats serum(CG, ASG, DXMG and KTG).72 hours later, collected the cells and supernatant. Cells were observed by electron microscope; Rho GDP dissociation inhibitors a (Rho GDIa) and Peripherin, relating to reconfiguration of cytoskeleton in AMCC, were detected in each group. And also the EPI in supernatant were quantified by ELISA. Result:As compared with the CG, the EPI in the cell supernate of the ASG(ASSG) was low significantly.(*P<0.05) As compared with the asthma group, the EPI in the cell supernate of the KTSG and DXMSG raised obviously(#P<0.05). With electron microscope result, Declined chromaffin granules, cytoplasm and mitochondria swelling and neurite were observed in ASSG, and compared with ASSG, the microstructural lesions in treatment groups(Dexamethasone and KTS) has relieved. As compared with the CSG, the Rho GDIa mRNA expression of the asthma group was lower significantly(*P<0.05), but the Peripherin mRNA expression was higher(#P<0.05), and the Rho GDIa fluorescence intensity of coal maceral was lower, but the Peripherin fluorescence intensity of coal maceral was higher. Compared with the ASSG, the Rho GDIa mRNA expression of the KTSG was higher significantly(ΔP<0.05), but the Peripherin mRNA expression was lower(οP<0.05), the Rho GDIa fluorescence intensity of coal maceral was higher, but the Peripherin fluorescence intensity of coal maceral was lower. Conclusion:1. the asthmatic rats serum can change the function and endocrine structure of the AMCC; 2. the kidney-tonfying recipe can protect the normal function and structure of AMCC, and suppress the decline of AMCC’s endocrine function.

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