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The Effect of Total Phenolics from Abnormal Savda Munziq on SiHa Cells

Author: ChenZuoYao
Tutor: GuZhaLiNuEr·ABuLiZi
School: Xinjiang Medical University
Course: Oncology
Keywords: Exception Black Munziq total phenolics SiHa cells Apoptosis FHIT Survivin
CLC: R29
Type: Master's thesis
Year: 2011
Downloads: 31
Quote: 1
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Abstract


Objective : To study the abnormal drug-dimensional black Munziq total phenolics SiHa cervical cancer cell lines in vitro proliferation and mechanism , thereby theoretically proposed exception black Munziq total phenol for the feasibility of the treatment of cervical cancer . Method: 1 ) with different concentrations of abnormal black Munziq total phenolics act on human cervical SiHa cells , in effect after 24h, 48h, 72h, 96h, using an inverted microscope morphological changes , MTT colorimetric assay each the inhibition rate of cells and half inhibitory concentration (IC50); 2) flow cytometry different concentrations of abnormal black Munziq total phenolics role in the human cervical carcinoma SiHa cell apoptosis after 48h rate changes , as well as Bcl -2 and telomerase expression ; 3 ) using real-time quantitative RT-PCR was used to detect different concentrations of abnormal black Munziq total phenolics role in the human cervical carcinoma SiHa cells 48 hours after the FHIT and Survivin gene expression . Results: 1 ) In 75-175μg/ml concentration range , unusual black Munziq total phenolics Shí SiHa cell proliferation significantly inhibited , and with the increase of drug concentration and treatment time , the inhibition rate l , the difference was statistically significant (P lt; 0.01); 2) SiHa cells were abnormal black Munziq total phenolics were apparent after effect characteristics of apoptosis , and with the drug concentration increased, the extension of time , the cells morphological changes more obvious ; 3 ) Flow cytometric analysis showed that with the exception of black Munziq total phenolic concentration increases , SiHa cell apoptosis rate increased , Bcl-2 and telomerase expression , the difference there was statistically significant (P lt; 0.05); 4) real-time quantitative RT-PCR results showed abnormal black Munziq total phenolics after , SiHa cells increased expression of FHIT , Survivin expression was decreased (P lt; 0.05). Conclusions: 1 ) abnormal black Munziq total phenol can inhibit the growth of cervical cancer SiHa cells , and showed a dose-response relationship and effect relationship ; 2 ) abnormal black Munziq total phenolics by inhibiting oncogene Bcl-2 telomerase and Survivin expression , and promote the expression of FHIT gene to induce apoptosis in SiHa

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