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Objective: To establish hindlimb ischemia in a rat model , observed the blood Xuefuzhuyutang treatment of limb ischemia , and of VEGF in ischemic tissue before and after treatment of CD34 gene expression changes in serum secreted by the endothelial cells of vasoactive substances endothelin (ET, Endothelin), nitric oxide (NO) content changes , to clarify the mechanism of the blood Xuefuzhuyutang treatment of limb ischemia disease . MATERIALS AND METHODS : Adult Wistar rats (200 ± 20g) 32 Zhi , made ??of hindlimb ischemia model , the random number table is divided into two large groups : the experimental group and model control group , each group then press the specimen collection time divided into surgery after two weeks , four weeks two subgroups , each subgroup are eight rats . Another 16 rats were used as normal controls . Experimental group , model control group at the same time modeling , control group received no treatment . Experimental rats modeling success simultaneously orally blood Xuefuzhuyutang , blood , respectively, at 14 and 28 days and take the right hindlimb adductor muscle tissue in the middle , to detect ischemic muscle expression of CD34 and VEGF positive cells model of the experimental group serum with the control group model serum NO and ET ??levels . Results: classic side blood circulation blood Xuefuzhuyutang can promote VEGF expression of lower limb ischemia model in rats , promoting lower limb ischemia model in rats CD34 antigen protein , of Xuefuzhuyu soup can improve lower limb ischemia big mouse serum , the content of NO to reduce the ET content . Conclusion : 1 . Blood Xuefuzhuyutang can effectively inhibit the release of ET , to promote the release of NO , to improved limb ischemia state inhibition in vivo local vasomotor abnormalities occurred . 2 . Blood Xuefuzhuyutang can raise the the rat the ischemic limb VEGF and CD34 expression , the promotion of the role of the ischemic limb angiogenesis .
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