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PDTC Prevents the Decrease of Insulin Gene Expression Induced by Elevated Glucose Concentrations in INS-1 Cell

Author: LiuZhiPing
Tutor: LiYuMing
School: Huazhong University of Science and Technology
Course: Internal Medicine
Keywords: Glucose toxicity Insulin PDX-1 MafA NF-κB PDTC
CLC: R587.1
Type: Master's thesis
Year: 2008
Downloads: 74
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Abstract


Objective To study the toxicity of high sugar INS-1 cell insulin gene transcription factor PDX-1 and MafA expression , as well as the case of high-sugar , NF-κB inhibitor PDTC ( pyrrolidine dithiocarbamate ) insulin , PDX -1 and MafA expression protection role , so as to explore the biochemical mechanisms of glucose toxicity may provide a new target for the treatment of diabetes . RPM1-1640 culture medium containing 11.1mmol / L glucose INS-1 cells , when the cells were grown to about 80% of fusion , and then , respectively, cells were transferred to contain 4.0mmol / L glucose (NG group ) , 16.7 mmol / L glucose ( HG group) and 16.7mmol / L glucose 50umol / L of PDTC ( Hg P group) in RPMI-1640 medium , culture was continued for 48 hours, the cells were collected by centrifugation , Trizol extracting total RNA , RT generated cDNA was then the real-time PCR were used to detect insulin gene transcription factor PDX -1 and MafA mRNA levels . The HG group NG group compared with the results of insulin mRNA expression decreased by 64.9% ( p lt; 0.05 ) ; PDX- 1 mRNA expression was decreased by 82.3% ( p lt; 0.01 ) ; MafA is the mRNA expression decreased by 80.9% (p lt; 0.01). HG P group compared with the HG group , insulin mRNA expression increased 6.67 -fold ( p lt; 0.05 ) ; PDX- 1 mRNA expression was increased 7.52 -fold ( p lt; 0.05 ) ; MafA is mRNA expression increased 5.70 -fold (p lt; 0.01); compared with the NG group , insulin , PDX-1 and MafA mRNA expression had no significant difference ( p gt ; 0.05) . Conclusion The high concentration of glucose can lead to decreased insulin gene and PDX-1 and MafA expression , NF-κB inhibitor PDTC can restore the insulin gene and PDX - 1 and MafA expression . The study results suggest that: \

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
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