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The Effects and Mechanisms of Glycyrrhetinic Acid on the Invasiveness and Migration Ability of Leukemic Cells

Author: ZhangJinLing
Tutor: ZouPing
School: Huazhong University of Science and Technology
Course: Internal Medicine
Keywords: Glycyrrhetinic acid K562 cells HL-60 cells Tumor invasion Matrix metalloproteinase
CLC: R733.7
Type: Master's thesis
Year: 2008
Downloads: 65
Quote: 2
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Abstract


Objective: To observe glycyrrhetinic acid (Glycyrrhetinic acid, GA) leukemia cell invasion and migration ability, and its mechanism. Method: select human myeloid leukemia cell K562, HL-60 cells for the study. MTT assay to detect different concentrations of GA role in K562 and HL-60 cells at different times after cell proliferation inhibition rate; of Transwell chamber matrix glue invasion experiments and chemokines experimental observation GA K562, of HL-60 cell line invasion and migration ability different concentrations of GA; semi-quantitative RT-PCR assay GA K562, HL-60 cell matrix metalloproteinases MMP-2 and MMP-9mRNA expression; detected by immunohistochemical staining of K562, HL-60 cells of MMP-2 and the impact of the level of expression of MMP-9 protein; of gelatin zymography GA on the two cell lines MMP-2 and MMP-9 activity. Results: 1.GA significantly inhibited K562 and HL-60 cell proliferation, and its effect was dose-dependent. 2 different concentrations of GA (30,60,120 μmol · L-1) K562, HL-60 cells after 24 hours, can significantly inhibit K562 and HL-60 cell invasion and migration, and a dose-dependent. The 3.GA role K562, HL-60 cells after 48 hours, can inhibit K562, HL-60 cells, MMP-2, MMP-9mRNA expression. K562 cells compared with the control group, 30μmol · L-1 GA of MMP-2mRNA expression was no significant difference (P gt; 0.05) 60,120 μmol · L-1 group significant sex difference (P lt; 0.05 in each group), MMP-9 and the control group than the difference was statistically significant (P lt; 0.05). HL-60 cells, compared with control group, MMP-2mRNA expression 30,60,120 μmol · L-1 group was statistically significant (P lt; 0.05). MMP-9 expression levels in each group significant difference (30μmol · L-1 group, P lt; 0.05; 60,120 μmol · L-group P lt; 0.01). 4. GA role of K562, HL-60 cells after 48 hours, can inhibit K562, HL-60 cells, MMP-2, MMP-9 protein expression. K562 cells compared with the control group, 30μmol · L-1 GA MMP-2, MMP-9 protein expression was not a significant difference (P gt; 0.05), 60,120 μmol · L-1 group significantly difference (P lt; 0.05);, MMP-2 expression in HL-60 cells, 60,120 μmol · L-1 with the control group compared the treatment group, a significant difference (P lt; 0.05); MMP-9 expression, GA concentration compared with control group showed a significant difference (P lt; 0.05). 5.GA inhibit K562, HL-60 cell line gelatinase activity in a dose-dependent manner. K562 cells appear only MMP-2 band, HL-60 cells to the MMP-2 and MMP-9 in the strip is detected. K562 and HL-60 cells, GA treatment group and control group comparison, 60,120 μmol · L-1 group showed significant differences (P lt; 0.05); 30μmol · L-1 group and the control group was not statistically significant ( P gt; 0.05). Conclusion GA inhibit K562 and HL-60 cell invasion and migration, likely by down-regulating MMP-2 and MMP-9mRNA and protein expression, and reduce its activity and to play a role.

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
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