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Comparison of the Volatile Oil from Fructus Piperis Longi by SD and SFE-CO2 and the Study on the Water Solution after Volatile Oil Extraction Refined by Membrane

Author: ZhangYuQiang
Tutor: GuoLiZuo
School: Nanjing University of Traditional Chinese Medicine
Course: Pharmacy
Keywords: Piper longum Steam distillation Supercritical CO2 Extraction Membrane separation Associated with
CLC: R284
Type: Master's thesis
Year: 2008
Downloads: 323
Quote: 0
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Abstract


The process of modernization of traditional Chinese medicine, the traditional separation methods are faced with challenges and opportunities. Chinese medicines substance refined target separation system, low concentration of raw materials, component complex and require a higher recovery rate. But the existing conventional separation techniques such as distillation, extraction, crystallization, adsorption, and ion exchange, etc., based on the concentration difference of the mass transfer driving force components to be separated by a high concentration to low concentration diffusion, often difficult to meet the above-mentioned separation systems requirements. From the trend of modern separation technology research and development, to address the problem from two aspects to solve: First, the study of new technologies for Chinese medicines material separation; second is the use of existing and newly developed separation The technology for the effective combination, or two or more separation techniques joint become a more efficient separation techniques, to achieve improved selectivity and yield of the product, to achieve the purposes of process optimization. The subject use the supercritical CO In recent years, more attention 2 extraction technology, membrane separation technology and gas chromatography - mass spectrometry technology, Piper longum the main pharmacodynamic component of the volatile oil The extraction process of the different extraction methods were selected and optimized, the analysis of the chemical composition of the volatile oil of Piper longum. Aims to explore different technologies and their effectiveness and relevance of the extraction and separation of combined application of Chinese herbal medicinal ingredients. The main research work: 1. HPLC method for quality control studies, Piper longum single flavor of herbs were collected from different habitats and batches of ingredients for the base source and Determination to ensure that qualified and controlled raw herbs for this thesis; factors influencing the stability of piperine (lighting, heating temperature and time, PH, etc.) and Piper longum extract (water extract, alcohol extract) in related research. 2 orthogonal design L 9 (3 4 ), steam distillation (SD) and ultra supercritical CO 2 extraction (SFE-CO 2 ) the two processes of volatile oil extract of Piper longum orthogonal test results of a comparative study. SD method to volatile oil extraction rate index, the optimal process for Piper longum the herbs soak 1 hour, plus 6 times the amount of water, the extraction time was 7 hours; SFE-CO 2 Law volatile oil extraction rate The composite score as an indicator of of piperine transfer rate, and the optimum process for the extraction pressure 20MPa, extraction temperature of 45 ° C, extraction time of 20min. By gas chromatography - mass spectrometry (GC-MS) technology, the chemical composition and the relative content of of Piper longum volatile oil extracted by SD method and SFE-CO 2 Law compared . Results of the volatile oil extracted by the two methods there are some differences in appearance, yield, chemical composition and its relative content. Piper longum SD method to extract the volatile oil was dark green cool transparent oily liquid extraction rate of 0.6%, while the SFE-CO 2 the Piper longum volatile oil extraction was the orange viscous semi-solid oil, extraction rate of 4.36%. TLC and HPLC method, two kinds of methods to extract in the dregs of Piper longum volatile oil piperine containing analysis and measurement extraction SFE-CO 2 the Piper longum remaining dregs enrichment piperine Piperine average content of 10.37mg / g, is much higher than the average content of the SD method extracted dregs piperine. Extracted for the SD method Piper longum volatile oil after water extraction in liquid systems, the use of inorganic ceramic membrane microfiltration membrane flux, medicinal components transfer rate on indicators, comprehensive survey of membrane structure parameters (membrane material, membrane pore size ) the applicability of the system and the process parameters (operating differential pressure, flow rate of the solid to liquid, operating temperature) on the impact of the film process. Optimization of the system parameters in accordance with the applicable research: the 0.2μm Aperture ZrO 2 ceramic microfiltration membranes, operation pressure 0.15MPa, solid to liquid flow rate of 3m / s, the operating temperature 50 ℃, the SD method to extract water Piper longum volatile oil mentioning the drug solution the microfiltration refined, and timely add water to the original volume. And analysis system for membrane separation of the aqueous extract liquid material foundation (common polymer) and physico-chemical parameters (pH value, conductivity, viscosity, etc.) before and after the change in trend, further validation of the relevance and effectiveness of the technology, this system refined . In this thesis research significance of: (1) use of modern extraction separation and detection techniques, systematic extraction, separation and analysis of Piper longum effective substances. (2) two or more joint applied to the active ingredients of the Chinese herbal medicine extraction and separation technology research, to explore the use of new technology for the complex system of traditional Chinese medicine refining process feasibility basis.

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