Dissertation > Excellent graduate degree dissertation topics show

Aqueous Two-phase/Three-liquid-phase Extraction of Natural Products

Author: ZhangHua
Tutor: XiuZhiLong
School: Dalian University of Technology
Course: Biochemical Engineering
Keywords: Aqueous two-phase three-liquid-phase extraction natural product bioactive component
CLC: TQ028.32
Type: Master's thesis
Year: 2009
Downloads: 821
Quote: 5
Read: Download Dissertation

Abstract


Bioactive components of natural products are extremely rich, including flavones, saponins, alkaloids, terpenes, coumarins, steroidal, quinones and other compounds. Solvent extraction is usually used for the extraction and separation of natural products on laboratorial and industrial scales. However, the separation efficiency of this method is low and its cost is high. Some technical problems in separation of bioactive components in natural products, such as Vaccinium uLiginosum Linn, Salia miltiorrhiza Bunge and Capsicum annuum L., were investigated in this work as follows:To begin with, the partition behavior of anthocyanidin, capsaicin and capsanthin was studied in an aqueous two-phase system (ATPS) composed of hydrophilic short-chain organic solvent and inorganic salt. The extraction conditions were determined and optimized. When ATPS was composed of 30% (w/w) ethanol and 19% (w/w) ammonium suLfate, the partition coefficient (K), recovery (R) and yield(Y) for anthocyanidins was 7.06, 94.3% and 91.2%, respectively. For capsaicin and capsanthin, the optimum condition was 20% (w/w) ethanol and 20% (w/w) dipotassium hydrogen phosphate ATPS. Compared with ethanol extraction containing 0.5% (w/w) sodium carbonate and thermal reflux by n-hexane, the yield of capsaicin and capsanthin was 78.1% and 20.5%, respectively. These resuLts demonstrated that aqueous two-phase extraction (ATPE) was effective for the extraction of strongly polar bioactive components in natural products.Next, three-liquid-phase extraction (TLPE) of tanshinone IIA and salvianolic acid B in Salia miltiorrhiza Bunge and capsaicin and capsanthin in Capsicum annuum L. was investigated by using a three-liquid-phase system (TLPS) composed of hydrophobic organic solvent / hydrophilic organic solvent/inorganic salt / water. The effect of hydrophobic organic solvent on partition of bioactive components in TLPS was determined under their optimum conditions of ATPE. Furthermore, the operation parameters of TLPE were optimized. When TLPS was composed of 25% (w/w) n-hexane, 60% (w/w) ethanol extract of Salia miltiorrhiza Bunge and 15% (w/w) ammonium suLfate, the partition coefficient (K) and recovery (R) of tanshinone IIA and salvianolic acid B was 2.74 and 134.82, 89.0% and 97.2%, respectively. For capsaicin and capsanthin, the optimum condition was 10% (w/w) n-hexane, 18% (w/w) ethanol, 18% (w/w) dipotassium hydrogen phosphate and 54% (w/w) water TLPS. In this system, capsanthin was enriched in the top phase (n-hexane phase), while capsaicin was mainly in the middle phase (ethanol phase). A higher yield couLd be got by TLPE than traditional extraction. The results revealed that different polar bioactive components exhibited different partition behavior in TLPS. Weakly polar components are enriched to the top phase, whiles strongly polar bioactive components are mainly in the middle phase and water-soluble impurities are in the bottom phase. According to difference in polarity, TLPE can effectively separate bioactive components in natural product, and simplify the latter purification step.Furthermore, the ATPE and TLPE had been scaled up taking Salia miltiorrhiza Bunge extract for example. When the ATPS and TLPS were scaled up to 400 times of the original system, the extraction efficiency showed little change. This illuminated that ATPE and TLPE were suited for the industrial production of bioactive components in natural product.Finally, ATPE combined with column chromatography was examined to purify anthocyanidins. TLPE combined with column chromatography was for purification of salvianolic acid B. A new process for separation of bioactive components in natural product was put forward: pretreatment→ATPE/TLPE→column chromatography→drying—product. Compared with traditional methods, the new one is a simple and integrated process with low cost. It is especially fit for industrial production of bioactive natural products.

Related Dissertations

  1. Research on Automatic Detection Algorithm for Substructure Distress of Highway Pavement Based on SVM,U418.6
  2. ISAR Imaging Simulation of Space Targets and Target Recognition Based on ISAR Images,TN957.52
  3. Research on Feature Extraction and Classification of Pulse Waveform for Cholecystitis and Nephrotic Syndrome Diagnosis,TP391.41
  4. Application of Q-Learning in the Content-Based Image Retrieval Technology,TP391.41
  5. Answer Extraction Based on Logic Representation and Reasoning for Reading Comprehension,TP391.1
  6. Research on Domain Entity Attribute and Event Extraction Technology,TP391.1
  7. Research on Transductive Support Vector Machine and Its Application in Image Retrieval,TP391.41
  8. Research on Feature Extraction and Classification of Tongue Shape and Tooth-Marked Tongue in TCM Tongue Diagnosis,TP391.41
  9. Research on Temporal Information Recognition and Normalization,TP391.1
  10. Research on Extraction and Tracking of People’s Opinion,TP391.1
  11. Multi-currency Notes Technology Research and Implementation,TP391.41
  12. The Research on Paper Currency Classification Method Based on Harr-Like Feature and Minimal Ball Including Samples,TP391.41
  13. Pavement Distress Recognition Based on Image,TP391.41
  14. Research on Visual Detection and Tracking of Mobile Robots,TP242.62
  15. Research on Fusion Algorithm of Hyper Spectral and High Spatial Resolution Remote Sensing Image,TP751
  16. Study on Extracting Process of Rhizoma Alismatis and Rhizoma Smilacis Glabrae and Their Hypouricemic Effects,R284
  17. Synthesis of Benzimidazolium Ionic Liquids and Their Application in Extraction Separation,O626.23
  18. Studies on Extraction and Analysis of Fructooligosaccharides in Smallanthus Sonchifolius,TS255.1
  19. An Approach for Identifying a Plant Resistance Gene Based on the Random Forest,Q943
  20. Phosphate byproduct sodium fluosilicate chlorine wastewater recycling study,X786
  21. Extraction, Purification and Antifungal Activity of Anthocyanins in Drop Fruits of Chinese Baberry,TQ914.1

CLC: > Industrial Technology > Chemical Industry > General issues > Chemical processes ( physical processes and physical and chemical processes ) > Separation process > Single-phase system liquid mixture separation process > Extraction (liquid - liquid extraction )
© 2012 www.DissertationTopic.Net  Mobile