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Asteraceae (Compositae) angiosperms, most chemical composition, the most complicated of the evolution Branch Artemisia Asteraceae strong insecticidal and antimicrobial activity, and wider distribution of a case in the Heilongjiang region . If both insecticidal and bactericidal activity of substances from Artemisia identify, undoubtedly of great significance. In this paper, Heilongjiang common species of Artemisia as the research object, its insecticidal activity and antibacterial activity of preliminary research. The main results are as follows. (1) insecticide trials first third instar larvae of the gypsy moth treatment, screening out the optimum extraction method, the results show that: insecticidal effect of crude extracts of five species using ultrasonic extraction method best cold soak method extraction of the crude extracts antifeedant best results. (2) toxicity and stomach toxicity test using ultrasonic extraction, poor feeding trials using cold soak extraction, different solvent extraction of the crude extracts of third instar gypsy moth larvae Toxicity test results are as follows: large seed Artemisia, Artemisia annua, scoparia has strong contact action, five species of Artemisia antifeedant effect. The crude extract concentration of 10mg/ml, tag the insecticidal activity of the crude extracts of chloroform to large seed Artemisia highest the 72h of corrected mortality was 73.33%. Stomach poison insect activity is generally not high, the highest scoparia acetone crude extract, of its 72h correction mortality of only 40.00%. Antifeedant activity on the solvent, the higher activity of chloroform. Plants, wild mugwort best, ethanol, chloroform, petroleum ether, acetone crude extract 24h antifeedant rate reached 68.98%, antifeedant rate of 75.84%, 78.58%, 70.26%, and 48 hours, respectively, 75.30%, 87.67%, 89.33%, 80.31%. (3) ultrasonic extraction conditions: extraction time, different solid-liquid ratio, extraction times, plant grinding fineness of the effects of forest pest have a certain impact test results on the effect of forest pest. Ultrasonic extraction time 40min appropriate; ultrasonic solid-liquid ratio of 1:20 is appropriate; ultrasonic extraction times 3 times better; grinding fineness 60 mesh is appropriate. (4) a light activation of the screening test results show that: the five species only the scoparia light activation properties. Scoparia contact and stomach poison and antifeedant tests showed insecticidal and antifeedant activity after the light treatment is better than after light treatment. (5) antibacterial test in the first extraction solvent and extraction method of screening results show that: Artemisia annua, Artemisia scoparia, wild mugwort optimum extraction method for ultrasonic extraction method, the large seed Artemisia optimum extraction method for cold immersion method, Mongolia Artemisia optimum extraction method for Soxhlet law; the large seed Artemisia, Artemisia annua optimum extraction solvent is ethanol scoparia wild mugwort, Mongolia Artemisia optimized extraction solvent acetone. (6) 5 species optimum extraction method and solvent extracted crude extracts of pathogen inhibition test results: large seed Artemisia annua, scoparia crude extract higher inhibition rate. Large seed Artemisia annua, Artemisia scoparia intends Cladosporium the Fusarium pathogens EC 50 were 32.44 mg / ml, 13.75 mg / ml, 13.85 mg / ml; sordida on Poplar Pathogen EC 50 were 9.88 mg / ml, 8.17 mg / ml, 9.58mg/ml, larch dieback disease pathogens EC 50 , respectively, to 20.67 mg / ml , 5.05 mg / ml, 5.98 mg / ml; corn leaf blight pathogens EC 50 , respectively 16.48mg/ml, 17.35 mg / ml, 12.85 mg / ml. (7) ultrasonic extraction conditions on the effects of the pathogen test results showed that: there is a certain concentration of different solvents, different extraction time, different solid to liquid ratio, extraction times pathogens effect. Artemisia annua, extraction solvent ethanol at a concentration of 70% is appropriate, extraction time 40min appropriate material to liquid ratio of 1:20 is appropriate extraction times, preferably three times; scoparia extraction time of 40min appropriate material liquid ratio 1:30 appropriate extraction times 3 is appropriate.
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