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Bioinformatics Study on Microbial in Low-Temperature Anaerobic Granular Sludge

Author: ZhuZuo
Tutor: LuoXiangNan
School: Hebei University of Science and Technology
Course: Environmental Engineering
Keywords: Low-temperature anaerobic Granular sludge Microbial strains Bioinformatics 16S rDNA Sequence analysis Phylogenetic tree
CLC: X172
Type: Master's thesis
Year: 2010
Downloads: 156
Quote: 0
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Abstract


Microorganism is the main function of biological wastewater treatment , the the various microorganisms coexist and interact in the wastewater treatment system . Fully aware of Microbiology principle can provide the basis for improving the treatment process to improve the processing efficiency . Based on traditional culture and purification technology has been unable to meet the researchers fast , accurate analysis and identification of the requirements of the Environmental Microbiology . Produce and become the core areas of the natural sciences in the 21st century , with the implementation of the Human Genome Project (HGP) , biological information learned through bioinformatics research platform , capable of real-time online to retrieve rich microbial resources , shared environmental microbial genome information . Use of 16S rDNA / rRNA sequence analysis technology , researchers can not microbes were isolated and cultured the case study , the analysis and identification of micro-organisms in a particular environment , and microbial community structure and function study , the results are accurate , fast. The subject get isolated and cultured rely on traditional methods of morphological , physiological and biochemical characteristics of the low - temperature anaerobic granular sludge microorganisms , 6 strains of 16S rDNA sequences obtained by 16S rDNA sequencing methods . And the use of new means of bioinformatics analysis of 16S rDNA sequences of strains . Nucleotide using the BioEdit software analysis sequence distribution , GC content, and by looking for an open reading frame of the nucleic acid sequence is translated into the corresponding amino acid sequence . 6 strain 16S rDNA sequence multiple sequence alignment using ClustalX software results based on the 6 strain 16S rDNA sequence homology and phylogenetic tree was constructed using the MEGA software , analysis of unknown bacteria and other bacteria kinds of kinship , further understanding of the evolutionary relationships of organisms .

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CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Biology > Environmental Microbiology
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