Dissertation > Excellent graduate degree dissertation topics show

Identification and Analysis of Microbial Community Structure for Gujing Liquor Daqu

Author: ShuYing
Tutor: ZhangZhiZhou
School: Harbin Institute of Technology
Course: Genetics
Keywords: GuJing Daqu microbe 16S rDNA phylogenetic tree species-specificamplification primers
CLC: TS261.1
Type: Master's thesis
Year: 2013
Downloads: 97
Quote: 0
Read: Download Dissertation

Abstract


In the traditional brewing process, Daqu determines the brewing quality to agreat extent. Different types of Daqu contain different microbial biodiversities. Thisstudy identified the microbial community composition of GuJing liquor Daqu basedon16S rDNA sequence analysis method. Meanwhile, the preliminary study of rapidquantification technology for Daqu species provided the necessary reference forfurther development of practical rapid quantitative techniques.This research is mainly composed of four parts:First, PCR amplification conditions were explored. Second, by using thetechnology of TA cloning and blue-white colony screening,16S rDNA clones werecollected, stored, replicated and sequenced. Third, all effectively sequenced cloneswere subject to Blast analysis to identify microbial species. Phylogenetic trees wereconstructed to display relationship among Daqu species. Fourth, several strategies torapidly quantify the main Daqu species were proposed, designed, and partiallyconfirmed by experiments.Conclusions obtained in this research are as follows:(1) GuJing Daqu microbial community was firstly characterized and analysed atthe molecular level. It is mainly composed of Firmicutes and Proteobacteria. Thereare six main dominant species, namely Virgibacillus, Bacillus, Lactobacillaceae,Pantoea, Staphylococcus and Thermoactinomycetaceae(2) By comparing microbe community structure of medium temperature Daqu.and high temperature Daqu, the former has a larger diversity.(3) By constructing phylogenetic tree, one potential new Bacillus species strainis found, namely strain of GJ-1-20in the medium temperature Daqu.(4) Through the initial study of rapid quantitative technology, we found that it isvery difficult to distinguish20or more species rapidily and quantitativly only on thebasis of these very similar16S rDNA sequences (around1500bp), even forTaqMan-MGB probe technology. Because most GuJing Daqu microbe genomes arenot expected to complete within the next ten years, the development of low-costsingle-base-resolution quantification technology only based on limited16S rDNAsequences is very worth waiting.

Related Dissertations

  1. Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer,Q938
  2. Isolation and Identification of Infectious Bronchitis Virus in Henan and the Full Genome Sequence Analysis of Isolated Strains HN104 and HN091,S852.65
  3. Identification、Diversity and Phylogenetic Analysis of Antagonistic Bacillus Isolated from Different Chinese Areas,S476.1
  4. Dalian Sea Screening and Identification of culturable marine actinomycetes,Q93
  5. Isolation and Purification of a Novel Antifungal Antibiotic and Optimization of Its Fermentation Conditions,TQ927
  6. Phylogenetic Study of Baculovirus Genome Based on Markov Model,Q939.4
  7. Three mild halophilic PAO Isolation, identification and characterization phosphorus,X172
  8. Genotypic Analysis of Hepatitis C Virus Isolated from Yantai of Shandong,R373
  9. Optimization of Fermentation Process for Exo-polysaccharides Production by Kombucha ZJU1 and the Application of Fermentation Broth,TS275
  10. Amplification and Sequence Analysis of HCV 1b Full-length Genome,R392.1
  11. Study on Isolation and Identification of Lactic Acid Bacteria in Fermented Camel Milk of Xinjiang and Its Bacteriocin,TS201.3
  12. Investigation on Bacterial Diversity in Portunus Trituberculatus Rearing Pond by Cultivation-dependent and Independent Approaches,S968.252
  13. Quantification of Human Intestinal Bacterial Combined cPCR and LDR,R440
  14. Study on the Antioxidation and Hypolipidemic Effects of Lactic Acid Bacteria,R285.5
  15. Study on Systematics of Root-nodules Actinomycetes,Q939
  16. Partial Nitrification Process of Sewage Treatment Technology of Ecological Control of Microorganisms,X703
  17. Isolation and Identification of Cellulase-Producing Bacterial Strains and Preliminary Studies on Conditions of Enzyme Production,S816
  18. The Characterization of the VP1 Gene and the 2A Gene, Protein of Enterovirus Type 71,R373
  19. Study on Diversity and Antibiotic Activity of Endophytic Isolated from Some Medicinal Plants,Q93
  20. Isolation and Identification of Bacterial Strains and Their Application in Deep-litter System,Q93
  21. Mapping B-cell Liner Epitopes of NS3 Protein of Bovine Viral Diarrhea Virus,S852.65

CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > Brewing industry > Brewing Industry > Microbes
© 2012 www.DissertationTopic.Net  Mobile