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Matrine is the main active ingredient of traditional Chinese medicine Sophora (Sophora flavescens Ait), Sophora has a long history in our country, its bitter, cold, a small drug, the heart, lung, kidney, large intestine meridians. The pharmacology study found matrine has a wide range of pharmacological activities, such as: analgesic, cardiac antiarrhythmic, anti-viral, anti-inflammatory, anti-tumor, swelling diuretic, immune suppression. Matrine mainly distributed in the body such as the kidney, liver, spleen, lung, brain and heart. Ceftiofur (Ceftiofur) is a third generation cephalosporin antibiotic, has a broad-spectrum antimicrobial activity on Gram-positive bacteria and negative bacteria as well as anaerobic pathogens. Ceftiofur in the body mainly in the kidney, lung, liver, fat and muscle. The mutual influence of matrine with hydrochloric acid ceftiofur rats in vivo pharmacokinetics of science, to explore the mechanism of action of the Chinese and western medicine and western medicine clinical medication reference. The experiment was divided into three groups, namely matrine administered alone, hydrochloric acid of ceftiofur administered alone group, of ceftiofur hydrochloride matrine administration group, experiments using high performance liquid chromatography determination of matrine, hydrochloric acid plasma concentration of ceftiofur matrine chromatographic conditions: column: Shim-pack VP-ODS 150Lx4.6 5μm; mobile phase: acetonitrile-0.02 mol · L-1 ammonium acetate aqueous solution - triethylamine (30:70:0.04, v / v / v); flow rate of 1mL · min-1; detection wavelength 220 nm; column temperature was 40 ° C; injection volume of 20 μL; chromatographic conditions, the matrine the detection limit is 200 ng · mL-1. The ceftiofur hydrochloride chromatographic conditions: Column: Shim-packVP-ODS 150Lx4.6, 5μm; mobile phase: acetonitrile -0.1% trifluoroacetic acid (30:70, V / v); velocity: 1mL · min- 1; detection wavelength 266 nm; column temperature: 40 ° C; injection volume of 20 μL; ceftiofur hydrochloride detection limit of 100 ng · mL-1 in this chromatographic conditions. Matrine treatment program with the DAS 2.1.1 pharmacokinetic plasma concentration - time data right. Ceftiofur hydrochloride by studying the pharmacokinetics of matrine rats to get the matrine separate administration group matrine Cmax 21.1139 mg · L-1, Tmax is 0.75 h, t1/2α to 1.34 The The h, t1/2β to 3.509 h AUCo ~ t 90.984 mg · h · L-1-8 AUCo 100.346 mg · h · L-1. Ceftiofur hydrochloride and matrine combined administration group matrine Cmax was 11.707 mg · L-1, Tmax 0.917 h of 1.598 h tl/2α, tl/2p to 3.247 h, 53.28 mg AUCo ~ t · h · L-1, AUCo-8 60.035 mg · h · L-1. The results show the blood concentration, bioavailability matrine matrine and ceftiofur hydrochloride when administered in combination significantly reduces the distribution of the outer circumferential chamber is reduced, the distribution of the central chamber is increased, the rate of in vivo clearance also increased. Matrine on the pharmacokinetics of ceftiofur hydrochloride in rats affect the outcome: ceftiofur hydrochloride administered alone ceftiofur hydrochloride Cmax of 24.311 mg · L-1, Tmax for 0.278 h, t1 / 2 0.371h, AUCo ~ t 26.847 mg · h · L-1, AUCo-8 29.021 mg · h · L-1. Ceftiofur hydrochloride and matrine combination group ceftiofur hydrochloride Cmax to 39.437 mg · L-1, Tmax 0.472 h, t1 / 2 for 0.777 h, AUCo ~ t 42.724 mg · h · L-1 , AUCo ~ 8 to 45.779 mg-HL-1. Shows that hydrochloric cephalosporins ceftiofur matrine combined plasma concentrations of ceftiofur hydrochloride administered significantly higher bioavailability, absorption rate decreases significantly longer half-life, decreased clearance rate. Ceftiofur hydrochloride matrine rat tissue distribution affect the results show that the co-administered group matrine in mind that the maximum concentration of the liver, spleen, lung, kidney, and brain were 20.02μg · g-1, 58.21μg · g-1, 103.68μg · g-1, 37.29μg · g-1, 167.00μg · g-1, 37.00μg · g-1. Administered alone compared with matrine, matrine increase in the amount of the distribution in the central compartment, have also been changes in the distribution of various organizations, namely the distribution of Matrine Matrine administered alone for kidney gt; liver gt; spleen gt; lung gt; brain gt; heart, co-administration of matrine distribution kidney gt; spleen gt; liver gt; lung gt; brain gt; heart. The ceftiofur hydrochloride results show that the impact of the of matrine mice analgesic effect, the co-administered group analgesic percentage (%) 56.16, compared with matrine administered alone group (36.92) has improved greatly. The the ceftiofur hydrochloride matrine in combination can significantly improve the analgesic effect of matrine and matrine separate administration group, effectively reduced acetic acid-induced writhing mice. It also further validates Ceftiofur hydrochloride administered in combination with matrine, matrine increased distribution in brain tissue.
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