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Anticoagulant Sulfated Fibroin Research

Author: LiShengChun
Tutor: XuShui
School: Southwestern University
Course: Special Economic Animal Feeding
Keywords: Sulfated fibroin Anticoagulant Biocompatibility
CLC: R318.08
Type: Master's thesis
Year: 2010
Downloads: 79
Quote: 3
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Abstract

Anti-clotting medicine and medical material has been widely used. If in the use of artificial dialysis and surgery to prevent blood clotting or in the preservation of blood within the tube to prevent blood coagulation. Many medical devices and artificial organs and other materials are also needed anticoagulant. In recent years, heparin, heparin, low molecular weight heparin, protease inhibitors, anti-platelet agents commonly used in the medical anticoagulant agents. Many medical devices and artificial organs materials and raw materials, mainly through blending these agents were prepared. However, because of their high cost, and have side effects, a need to develop a novel anti-clotting material. The study found that, SO ₄ ^{2 - for the anticoagulant effect of heparin in an important group, so if the introduction of SO ₄ ^{2 - , also can produce the similar structure of heparin, the anticoagulant effect can be given. Developed in recent years have been synthesized a sulfated polymer or polysaccharide, they have good anticoagulant effect, the biocompatibility is relatively good, but relatively poor stability and difficult materials for biomedical . This compares with fibroin has many advantages, such as good oxygen permeability, breathable, and other unique physical and chemical properties and biocompatibility for protein material, it is easy to give additional functionality using protein engineering. Sulfated fibroin functions found to study prospects getting better. In this study, in order to simplify the preparation of sulfated fibroin were prepared anticoagulant effects and good biocompatibility sulfated fibroin for the purpose of cocoon shell as raw materials, the use of pyridine-sulfur trioxide to prepare sulfated fibroin and determination of its sulfur content, anticoagulant effects and biocompatibility targets. The main results are as follows: 1. Soluble chemical methods for preparing fibroin cocoon shell with 10g prepared after treatment 6.5g soluble silk fibroin. Physical methods, the average per head of silkworm silk fibroin soluble extract O.1g. Both methods derived soluble silk fibroin is substantially the same in structure. In the selection of raw materials preparation, prepared according to the nature of the soluble silk fibroin and practical needs of the method you choose. (2) Preparation of sulfated fibroin with time and pyridine sulfur trioxide increases the quality, the color is gradually deepened. With the addition of pyridine sulfur trioxide quality and the increased processing time, the molecular weight of fibroin gradually becomes smaller, the resulting fibroin the amount of sulfated corresponding reduction. To get more samples and ensure the quality of the sample, so to choose an optimal experimental conditions. 3 Detection of sulfated fibroin pyridine sulfur trioxide 5g more than 2h samples processed by infrared spectroscopy SO ₄ ^{2 - can be grafted onto the wire vegetarian. When adding an amount of sulfur trioxide pyridine 10g, the processing time is 6h, the sulfated fibroin maximum sulfur content of the sample, was 1.26 mmol / g. 4 sulfated fibroin anticoagulant effect detection dubbed the best conditions of different concentrations of sulfated fibroin solution test, 3 min within the silk fibroin and 0.2 mg / mL sulfated fibroin tubes of blood has been condensed; 30 min after , 0.02 mg / mL heparin and 0.4,0.6 mg / mL sulfated fibroin tube of blood also condenses; 1h after, 0.04 mg / mL heparin solution tube of blood coagulation also, and 0.8 mg / mL sulfated silk Su is showing significant anti-clotting effect. 5 biocompatibility testing according to national GB / T 16886 provides methods detected, sulfated fibroin contained pyrogen compliance, did not cause skin sensitization reactions, integrity and damaged skin without local irritation , non-toxic reactions and hemolytic reaction phenomena.}}}

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