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Objective: endoplasmic reticulum stress (ERS) signaling pathway as endogenous defense system and disease incidence has important physiological and pathological significance. Sports Muscle Injury process, there will be varying degrees of oxidative stress, and oxidative stress will cause ERS is currently unknown. Therefore, following the slope of this article ran motion induced skeletal muscle injury, explore the ERS changes provide new ideas for to reveal EIMD mechanism and promote damage repair. PATIENTS AND METHODS: 72 8-week-old male SD rats were randomly divided into a control group and the exercise group. Exercise group were divided into 0h group after exercise, after exercise 6h group, 12h group after exercise, 1d group after exercise, after exercise 2d, 3d group after exercise, the 1w group after exercise and after exercise 2w group, n = 8 . Formal experiment, the exercise group animals once persistent downhill running speed of 16m/min, the gradient of -16 °, the total exercise time of 90 min. After the end of the movement, in 0h, 6h, 12h, 1d, 2d, 3d, 1w, 2w after death. The rat right gastrocnemius conventional HE staining complete histological detection and complete the endoplasmic reticulum stress protein detection, simultaneous determination of changes of SOD and MDA western blot experiment. Photoshop and Scion Image image analysis software for image acquisition and analysis and processing, using SPSS17.0 statistical software to analyze the data. Results: (1) organizational learning the results of the performance: the quiet control group muscle cells arranged in neat, rules, and after exercise 0h not found significant morphological change 6h discovered swelling of the muscle fibers, 12h muscle fibers continued swelling, 1d found that part of the muscle fiber rupture 2d group of inflammatory cells was significantly increased, 3d found that a large number of inflammatory cell infiltration, and the degree of injury is the most serious and, 1w occasionally inflammatory cell infiltration, muscle fiber morphology after 2w basically returned to normal. (2) changes of SOD performance after exercise 0h peak, after reducing 1d, 3d and 2w are the lowest. MDA changes in performance peak 0h after exercise, and thereafter continued to decline, 2w lower than the control group. SOD / MDA changes in performance is decreased and then increased, were lower than the quiet control group, which meet the minimum 1d after exercise. (3) GRP78 and GADD153 and the ratio change performance: persistently elevated after exercise declined, but when the 2d, 3d and 1w after rising 2w down, but still higher than the quiet control group. Conclusion: (1) first decreased skeletal muscle SOD / MDA changes after downhill running rise 1d reach the minimum after exercise, indicating that the repair of skeletal muscle injury early antioxidant capacity decline. Endoplasmic reticulum stress protein expression after exercise 1d reach a small peak, indicating that the endoplasmic reticulum stress may be activated by oxidative stress. (2) endoplasmic reticulum stress protein movement 2d decline, 3d peak again, combined with histological changes, suggesting late in the skeletal muscle injury repair, endoplasmic reticulum stress may play a positive role in the repair of skeletal muscle .
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