Dissertation > Excellent graduate degree dissertation topics show

A Study on Extraction of Amygdalin and Protein Powder from Amygdalus Pedunculatus Pall

Author: LiuPing
Tutor: ShenZuoHua
School: Northwestern University
Course: Analytical Chemistry
Keywords: Amygdalus pedunculatus pall amygdalin protein powder extraction
CLC: TS255.1
Type: Master's thesis
Year: 2010
Downloads: 262
Quote: 2
Read: Download Dissertation

Abstract


China is one of the largest desertification countries in the world. Governance and control of land desertification and dust storms occurrence have become an urgent environmental problem. Amygdalus pedunculatus pall is a new type of biomass energy material, with strong desert viability and sand control, being rich in nutritional composition in kernel, and higher economic value. After testing, Amygdalus pedunculatus pall kernel has abundance of amygdalin and protein, which were determined to be 3.73% and 25.2%, respectively. In order to achieve comprehensive utilization of Amygdalus pedunculatus pall, extraction of amygdalin and protein powder, and analysis of nutritional composition of the products and the final residues were studied.This thesis includes the following three components:1. Extraction of amygdalinThe extraction process of amygdalin from Amygdalus pedunculatus pall kernel was carried out in experimental condition. The single factor test and orthogonal test results showed that the optimum conditions were liquid ratio 1:9 (w/v) by adding ethanol, extraction temperature 75℃, extraction time 65 min; After extraction solution evaporated to dry, liquid ratio 1:30 (w/v) by adding 95% ethanol to the crude products, then crystallized 24 h to obtain purified amygdalin products. The purity of amygdalin was 96.20% measured by High Performance Liquid Chromatography (HPLC), the total yield was 43.07%. Melting point, UV, IR, NMR spectra were also used to identify the product structure.2. Extraction of protein powder and evaluation of nutritional valueThe extraction process of protein powder from the residues was studied in experimental and amlplification for the first time, and contents of the general composition of the products, amino acids, vitamins, mineral elements were determined. The results of single factor and orthogonal test showed that the optimum conditions at small scale were liquid ratio 1:25 (w/v) by adding water, pH 10.5, extraction temperature 60℃, extraction time 45 min, pH being adjusted to 4.5 to precipitate protein, then dried to obtain Amygdalus pedunculatus pall protein powder products. The amplified small scale to 90 times test results showed that the total yield was 40.6%, protein content more than 95%, fat content 0.18%, rich in nutritional composition-amino acids, vitamins and mineral elements, and thus can be regarded as a new nutritional product with low fat and high protein; quality security needs further study.3. Analysis of the final residuesThe general composition, amino acids and mineral elements of the final residue were also analysized after extraction. The experimental results showed that the final residues’ moisture was 7.62%, ash 8.38%, crude fat 16.50%, crude protein 33.70%; containing 18 kinds of amino acids existed in plants, total amino acids 33.65 g/100g,8 kinds of essential amino acids 10.46 g/100g; still having large quantity of mineral elements. The final residues can be used as an animal feed.

Related Dissertations

  1. Research on Automatic Detection Algorithm for Substructure Distress of Highway Pavement Based on SVM,U418.6
  2. ISAR Imaging Simulation of Space Targets and Target Recognition Based on ISAR Images,TN957.52
  3. Research of Question Answering System Based on the Analysis of Lexical and Semantic Meanings,TP391.1
  4. Research on Feature Extraction and Classification of Pulse Waveform for Cholecystitis and Nephrotic Syndrome Diagnosis,TP391.41
  5. Application of Q-Learning in the Content-Based Image Retrieval Technology,TP391.41
  6. Answer Extraction Based on Logic Representation and Reasoning for Reading Comprehension,TP391.1
  7. Research on Domain Entity Attribute and Event Extraction Technology,TP391.1
  8. Research on Transductive Support Vector Machine and Its Application in Image Retrieval,TP391.41
  9. Research on Feature Extraction and Classification of Tongue Shape and Tooth-Marked Tongue in TCM Tongue Diagnosis,TP391.41
  10. Research on Temporal Information Recognition and Normalization,TP391.1
  11. Context-Dependent Lexical Paraphrasing,TP391.1
  12. Research on Extraction and Tracking of People’s Opinion,TP391.1
  13. The Study of Domain Dictionary Construction Based on Web,TP391.1
  14. Research on Visual Measurement for Spacecraft Rendezvous and Approach,TP391.41
  15. Research on the Image Real-Time Acquisition, Storage and Image Processing System,TP391.41
  16. Feature Extraction, Selection and Combination in Lipreading,TP391.41
  17. Multi-currency Notes Technology Research and Implementation,TP391.41
  18. The Research on Paper Currency Classification Method Based on Harr-Like Feature and Minimal Ball Including Samples,TP391.41
  19. Pavement Distress Recognition Based on Image,TP391.41
  20. Research on Visual Detection and Tracking of Mobile Robots,TP242.62
  21. Research on Fusion Algorithm of Hyper Spectral and High Spatial Resolution Remote Sensing Image,TP751

CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > Fruits, vegetables,nuts processing industry > Basic science
© 2012 www.DissertationTopic.Net  Mobile