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Effects of Artesunate on the Biological Function of Hepatocellular Carcinoma HepG2 Cells

Author: LiuZhiLong
Tutor: CaoMingRong
School: Jinan University
Course: Surgery
Keywords: Artesunate HePG2 cells Hepatic carcinoma Apoptosis Drug sensitivity
CLC: R735.7
Type: Master's thesis
Year: 2010
Downloads: 54
Quote: 0
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Abstract


Objective: To study artesunate (artesunate, ART) digestive tumors from HepG2 role in cell proliferation and apoptosis, and the detection sensitivity of the impact of human hepatoma HepG2 cells to chemotherapeutic drugs for further clinical research and application basis. Method: 1. WST-8 was observed by different concentrations of artesunate on human hepatoma HepG2 cells, human colon cancer LoVo cell growth and proliferation. 2. Inhibition of colony formation detection of artesunate on human hepatoma HepG2 cell colony formation. 3.Hoechest 33258 staining observed the artesunate effect morphological changes of apoptosis in human hepatoma HepG2 cells. 4.PI staining the flow cytometry artesunate on human hepatoma HepG2 cell cycle and hypodiploid rate. 5.Annexin V-PI double staining Determination of artesunate on human hepatoma HepG2 cells apoptosis. 6.WST-8 assay artesunate chemosensitivity of human hepatoma HepG2 cells. Results: 1. Of artesunate role in human hepatoma HepG2 cells, human colon cancer LoVo cells after 48h, effectively inhibited HepG2 cells, Lovo cell proliferation, with the increase of concentration, the higher the rate of value-added inhibition, IC50 value, respectively 19.2umol / l, 68.3umol / l. 2 the Artesunate role in human hepatoma HepG2 cells after 7 days, the number of clones can effectively inhibit the formation of HepG2 cells. 3. Of artesunate role in human hepatoma HepG2 cells after 48h, Hoechest 33258 staining the experimental group cell nuclei pyknosis, margination and lysis, formation of apoptotic bodies apoptotic morphological changes. 4. Of artesunate role in human hepatoma HepG2 cells 48 h PI single dye flow cytometry cell cycle experimental group cells were arrested at G2 phase. 5. Of artesunate role in human hepatoma HepG2 cells 48 h after the PI single dye assay experimental group hypodiploid apoptosis peak; Annexin V-PI double staining experiments testing the experimental group cells appear early withered The death cell populations. The artesunate combination chemotherapy drug 5 - fluorouracil (5-fluorouracil ,5-Fu), carboplatin (carboplatin), epirubicin than the star (epirubicin) role in human hepatoma HepG2 cells after 48h, can significantly enhance the chemotherapeutic drugs on inhibition of tumor cells. Conclusion: 1. Artesunate on human hepatoma HepG2 cells, human colon cancer the Lovo cells have inhibited the proliferation. 2 artesunate able human hepatoma HepG2 cell cycle arrest in the G2 phase, and induce apoptosis. Artesunate can improve the human hepatoma HepG2 cells to 5-FU, carboplatin, epirubicin sensitivity.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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