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In recent years , an organic phosphorus compound , such as methyl parathion, due to high toxicity , environmental persistence and low Alternate organic chlorine widely used as a pesticide in agricultural production . Organophosphorus pesticide use range from pesticides extended to a variety of purposes such as fungicides, herbicides , rodenticides , resulting in the environment, groundwater, surface water , such as water and soil pollution , and serious residues in food threat to human health . Therefore , developed a rapid detection of organophosphorus pesticides , reliable, sensitive and practical methods of analysis , has a vital role in human health and ecological balance . The conventional method for detection of organophosphorus pesticides tedious pretreatment of the sample , the high cost of the required equipment is expensive and cumbersome , and the need for professional maintenance , not suitable for on-site testing . PDMS microfluidic chip used sample dose is less easy to detect , the analysis time is short , simple equipment , widely used in a variety of biological and chemical occasions . Successful system to a method for the detection of organophosphorus pesticides microfluidic chip , microarrays fiber and organophosphorus hydrolase , microfluidic channel integrated in the base material of polydimethylsiloxane ( PDMS ) . Inside in the microfluidic channel by the photolithography technology to produce a large number of cylindrical array of SU-8 , the organophosphorus hydrolase adsorption fixed manner in a cylindrical array , immobilized enzyme hydrolysis of the organic phosphorus , depending on the reaction product of the absorption of light to achieve the organophosphorus pesticide for detection. The results showed a good linear relationship ( correlation coefficient of 0.99) of the device at 10-6 ~ 10-1mol / L within . Also built experimental platform for detecting light absorption , and the general volume of organophosphorus pesticide experiments . The study of enzyme solution state and the state where the fixed enzyme hydrolysis reaction studied in the experimental results under different buffer . In addition, we also investigate the process of PDMS microfluidic microarrays . Study SU -8SU - Motherboard preparation process , PDMS curing conditions , as well as PDMS , and the type of substrate bonding method .
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