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Technique of Tissue Culture and Rapid Propagation of Rhododendron Chrysanthum Pall and Analysis of Effective Constituent

Author: YuQiuYan
Tutor: CaoHouNan
School: Yanbian University
Course: Pomology
Keywords: Rhododendron Tissue culture Domestication Determination of active ingredients
CLC: S685.21
Type: Master's thesis
Year: 2010
Downloads: 150
Quote: 0
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Abstract


Rhododendron distributed in China is very narrow , only in the Changbai Mountain area distribution . Because of its high ornamental value and medicinal value , great development potential . In order to increase the the Rhododendron population , and the development and application of cultivation and domestication and medicinal value , the experimental Rhododendron seed as test materials , study the Tissue Culture ; HPLC method with UV spectrophotometry were measured in Changbai Mountain Rhododendron tissue culture and wild seedlings medicinal ingredients rutin and tannic acid content . The main findings are as follows : 1 Rhododendron seed embryo is more appropriate for disinfecting compositions with 75% alcohol disinfection 30s , 0.1 % mercuric chloride disinfection 3min ; the suitable initial medium for Read GA32.0mg -1 , the germination rate of 8.8% ; 2 more suited Rhododendron subculture medium for the improved MS ZT1.0mg · L , - 1 IBA0.5 mg · L -1 , the multiplier effect is best , cultured for 40 days after the proliferation of multiple and increasing height , respectively, 5.04 and 1.56cm ; 3 more suited Rhododendron leaves callus induction medium for the improved MS TDZ1.0 mg L -1 2,4- D0 .3 mg · L - 1 dark culture 15d , callus rate of 100% ; compared with suitable for callus medium was changed MS TDZ1.5 mg · L - 1 NAA0.05 mg · L -1 , differentiation rate of 95% . 4 more suited Rhododendron plantlets the tube rooting medium was 1/4 improved MS IBA1.5 mg · L - 1 sucrose 20 g · L -1 , the rooting rate reached 100% . 5 Rhododendron tissue culture hardening transplant survival is the first exercise in the sand for 10 days, and then transplanted into humus soil : peat soil : sand = 2:1:1 matrices , the survival rate of 90 % . 6 the Rhododendron security wintering mode casing plant whole plant to ensure that no winter chilling injury . 7 Rhododendron wild seedlings rutin and tannic acid were higher than in tissue culture .

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Flower trees in class > Azaleas
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