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Background Systemic lupus erythematosus (Systemic lupus erythematosus, SLE) is a body can affect multiple organs and systems autoimmune diseases, clinical manifestations complex pathogenesis is not clear. Currently believed that genetic, immune, viral infections, drugs, hormones and environmental factors, including genetic pathogenesis of SLE plays an important role in nearly two decades on the rapid development of SLE genetics. The research team mainly through linkage analysis and candidate gene approach and lupus mouse model of SLE susceptibility genes, Online Mendelian Inheritance in Man (Online Mendelian Inheritance of human, OMIM) database contains 13 SLE susceptibility genes / loci, respectively, 1q41-q42 (SLEB1), 2q37.3 (SLEB2), 4p16-p15.2 (SLEB3), 12q24 (SLEB4), 13q32 (SLEB5), 16q11.2 (SLEB6), 20p12 (SLEB7), 20q13.1 (SLEB8), 1q32 (SLEB9), 7q32 (SLEB10), 2q32.2-q32.3 (SLEB11), 8p23.1 (SLEB12) and 6p23 (SLEB13), of which the latter two susceptibility genes / loci is to use the full Genome-wide association analysis method obtained. Our group conducted in Han population GWS studies also found nine new SLE susceptibility genes / loci, including ETS1, IKZF1, RASGRP3, SLC15A4, TNIP1, 7q11.23, 10q11.22, 11q23.3 and 16p11.2 (1.77 × 10? 25 ≤ P ≤ 2.77 × 10? 8). SLE is a complex disease that was non-Mendelian inheritance pattern, with a strong ethnic differences and genetic heterogeneity, to explore the pathogenesis of SLE ETS1 gene's role, this study in 61 patients with SLE peripheral blood mononuclear cells The ETS1 gene expression were detected, to investigate its role in the occurrence of SLE. Objective To study the ETS1mRNA in SLE patients peripheral blood mononuclear cells (PBMC) real-time quantitative expression levels, as well as SNPrs6590330 with ETS1 mRNA expression between. Methods 61 cases of SLE patients and 67 normal control subjects clinical data, peripheral blood total RNA was extracted and reverse-transcribed into cDNA, real-time fluorescence quantitative polymerase chain reaction (PCR) was used to detect the patient group and the control group ETS1mRNA Quantitative differences in expression levels; application Sequenom MassArray technique in 61 patients with SLE ETS1 genes rs6590330 locus were genotyped using SPSS10.0 statistical software for data analysis. Results in patients with SLE ETS1mRNA expression level of 0.3240 ± 0.1137, lower than healthy controls expression levels in peripheral blood ETS1mRNA 0.4128 ± 0.151, compared with normal subjects, SLE patients was ETS1mRNA expression level difference was statistically significant (P lt; 0.01); rs6590330 locus genotypes ETS1 between peripheral blood mononuclear cells showed no significant difference. Conclusion ETS1 gene may be associated with the pathogenesis of SLE.
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